Dissecting the potential role of hepatitis E virus ORF1 nonstructural gene in cross-species infection by using intergenotypic chimeric viruses

被引:9
作者
Tian, Debin [1 ]
Yugo, Danielle M. [1 ]
Kenney, Scott P. [2 ]
Heffron, C. Lynn [1 ]
Opriessnig, Tanja [3 ,4 ,5 ]
Karuppannan, Anbu K. [5 ]
Bayne, Jenna [5 ]
Halbur, Patrick G. [5 ]
Meng, Xiang-Jin [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Virginia Maryland Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA
[2] Ohio State Univ, Food Anim Hlth Res Program, Dept Vet Prevent Med, Coll Vet Med, Wooster, OH USA
[3] Univ Edinburgh, Infect & Immun Div, Roslin Inst, Edinburgh, Midlothian, Scotland
[4] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh, Midlothian, Scotland
[5] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA
基金
英国生物技术与生命科学研究理事会; 美国国家卫生研究院;
关键词
cross-species infection; hepatitis E virus (HEV); host tropism; intergenotypic chimeric viruses; pig; zoonotic infection; MUTATIONAL ANALYSIS; PROTEIN; GENOTYPE; CELLS; REPLICATION; HEV; DISCOVERY; SEQUENCES; CONTAINS; REQUIRES;
D O I
10.1002/jmv.26226
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis E virus (HEV) infects humans and more than a dozen other animal species. We previously showed that open reading frame 2 (ORF2) and ORF3 are apparently not involved in HEV cross-species infection, which infers that the ORF1 may contribute to host tropism. In this study, we utilize the genomic backbone of HEV-1 which only infects humans to construct a panel of intergenotypic chimeras in which the entire ORF1 gene or its functional domains were swapped with the corresponding regions from HEV-3 that infects both humans and pigs. We demonstrated that the chimeric HEVs were replication competent in human liver cells. Subsequently, we intrahepatically inoculated the RNA transcripts of chimeras into pigs to determine if the swapped ORF1 regions confer the chimeras' ability to infect pigs. We showed that there was no evidence of infectivity in pigs for any of the chimeras. We also investigated the role of human ribosome protein sequence S17, which expanded host range in cultured cells, in HEV cross-species infection. We demonstrated that S17 insertion in HEV ORF1 did not abolish HEV replication competency in vitro, but also did not expand HEV host tropism in vivo. The results highlight the complexity of the underlying mechanism of HEV cross-species infection.
引用
收藏
页码:3563 / 3571
页数:9
相关论文
共 54 条
  • [21] Identification and Fine Mapping of Nuclear and Nucleolar Localization Signals within the Human Ribosomal Protein S17
    Kenney, Scott P.
    Meng, Xiang-Jin
    [J]. PLOS ONE, 2015, 10 (04):
  • [22] The Lysine Residues within the Human Ribosomal Protein S17 Sequence Naturally Inserted into the Viral Nonstructural Protein of a Unique Strain of Hepatitis E Virus Are Important for Enhanced Virus Replication
    Kenney, Scott P.
    Meng, Xiang-Jin
    [J]. JOURNAL OF VIROLOGY, 2015, 89 (07) : 3793 - 3803
  • [23] Genetic host specificity of hepatitis E virus
    Lara, James
    Purdy, Michael A.
    Khudyakov, Yury E.
    [J]. INFECTION GENETICS AND EVOLUTION, 2014, 24 : 127 - 139
  • [24] Chronic Infection With Camelid Hepatitis E Virus in a Liver Transplant Recipient Who Regularly Consumes Camel Meat and Milk
    Lee, Guan-Huei
    Tan, Boon-Huan
    Teo, Esmeralda Chi-Yuan
    Lim, Seng-Gee
    Dan, Yock-Young
    Wee, Aileen
    Aw, Pauline Poh Kim
    Zhu, Yuan
    Hibberd, Martin Lloyd
    Tan, Chee-Kiat
    Purdy, Michael A.
    Teo, Chong-Gee
    [J]. GASTROENTEROLOGY, 2016, 150 (02) : 355 - +
  • [25] Genotype 5 Hepatitis E Virus Produced by a Reverse Genetics System Has the Potential for Zoonotic Infection
    Li, Tian-Cheng
    Bai, Huimin
    Yoshizaki, Sayaka
    Ami, Yasushi
    Suzaki, Yuriko
    Doan, Yen Hai
    Takahashi, Kazuaki
    Mishiro, Shunji
    Takeda, Naokazu
    Wakita, Takaji
    [J]. HEPATOLOGY COMMUNICATIONS, 2019, 3 (01) : 160 - 172
  • [26] Virus-specific mRNA capping enzyme encoded by hepatitis E virus
    Magden, J
    Takeda, N
    Li, TC
    Auvinen, P
    Ahola, T
    Miyamura, T
    Merits, A
    Kääriäinen, L
    [J]. JOURNAL OF VIROLOGY, 2001, 75 (14) : 6249 - 6255
  • [27] Hepatitis E virus-associated cryoglobulinemia in solid-organ-transplant recipients
    Marion, Olivier
    Abravanel, Florence
    Del Bello, Arnaud
    Esposito, Laure
    Lhomme, Sebastien
    Puissant-Lubrano, Benedicte
    Alric, Laurent
    Faguer, Stanislas
    Izopet, Jacques
    Kamar, Nassim
    [J]. LIVER INTERNATIONAL, 2018, 38 (12) : 2178 - 2189
  • [28] Expanding Host Range and Cross-Species Infection of Hepatitis E Virus
    Meng, Xiang-Jin
    [J]. PLOS PATHOGENS, 2016, 12 (08)
  • [29] From barnyard to food table: The omnipresence of hepatitis E virus and risk for zoonotic infection and food safety
    Meng, Xiang-Jin
    [J]. VIRUS RESEARCH, 2011, 161 (01) : 23 - 30
  • [30] Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV
    Meng, XJ
    Halbur, PG
    Haynes, JS
    Tsareva, TS
    Bruna, JD
    Royer, RL
    Purcell, RH
    Emerson, SU
    [J]. ARCHIVES OF VIROLOGY, 1998, 143 (07) : 1405 - 1415