Brassica oleracea L. var. botrytis Leaf Extract Alleviates Gentamicin-Induced Hepatorenal Injury in Rats-Possible Modulation of IL-1β and NF-κB Activity Assisted with Computational Approach

被引：6

作者：

Khalil, Hany Ezzat ^{[1
,2
]}

Abdelwahab, Miada F. ^{[2
]}

Emeka, Promise Madu ^{[1
]}

Badger-Emeka, Lorina, I ^{[3
]}

Ahmed, Al-Shaimaa F. ^{[4
]}

Anter, Aliaa E. ^{[4
]}

Hafez, Sara Mohamed Naguib Abdel ^{[5
]}

AlYahya, Khalid A. ^{[6
]}

Ibrahim, Hairul-Islam Mohamed ^{[7
,8
]}

Thirugnanasambantham, Krishnaraj ^{[8
,9
]}

Matsunami, Katsuyoshi ^{[10
]}

Selim, Alyaa Hatem Ibrahim ^{[11
]}

机构：

[1] King Faisal Univ, Coll Clin Pharm, Dept Pharmaceut Sci, Al Hasa 31982, Saudi Arabia

[2] Minia Univ, Fac Pharm, Dept Pharmacognosy, Al Minya 61519, Egypt

[3] King Faisal Univ, Coll Med, Dept Biomed Sci, Al Hasa 31982, Saudi Arabia

[4] Minia Univ, Fac Pharm, Dept Pharmacol & Toxicol, Al Minya 61519, Egypt

[5] Minia Univ, Fac Med, Dept Histol & Cell Biol, Al Minya 61519, Egypt

[6] King Faisal Univ, Coll Med, Dept Surg, Al Hasa 36363, Saudi Arabia

[7] King Faisal Univ, Coll Sci, Dept Biol Sci, Al Hasa 31982, Saudi Arabia

[8] Pondicherry Ctr Biol Sci & Educ Trust, Pondicherry 605004, India

[9] Saveetha Inst Med & Tech Sci, Saveetha Sch Engn, Dept Biotechnol, Chennai 602105, Tamil Nadu, India

[10] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Pharmacognosy, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan

[11] Sohag Univ, Fac Pharm, Dept Pharmacognosy, Sohag 82524, Egypt

来源：

LIFE-BASEL | 2022年 / 12卷 / 09期

关键词：

Brassica oleracea; cauliflower; gentamicin; GSH; AST; creatinine; IL-1; beta; NF-kappa B; INDUCED NEPHROTOXICITY; OXIDATIVE STRESS; INDUCED TOXICITY; ANTIOXIDANT; INFLAMMATION; BROCCOLI; KIDNEY; VALORIZATION; ACTIVATION; APOPTOSIS;

D O I：

10.3390/life12091370

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Recently, crop byproducts are considered a hot topic and can be converted into beneficial products. Cauliflower is well-known for its protective effects against oxidative stress-induced damage. The current study aimed to investigate the chemical profile and the ameliorative effects of cauliflower leaf extract (CL) on gentamicin-induced renal and hepatic injuries in rats. Methods: Cauliflower leaf was extracted with methanol to give the total methanol extract (TME) followed by the determination of total phenolic contents (TPC). Rats were divided into five groups; Group I was assigned as the control group, while the other groups were injected with gentamicin for ten days. Group II was given distilled water. Rats in groups III and IV were treated with oral CL (200 mg/kg and 400 mg/kg, respectively). Group V received L-cysteine (as a positive control). The functions of the kidneys and liver; oxidative stress and morphological and apoptotic changes of renal and hepatic tissues were assessed. Results: The TME was subjected to chromatographic techniques to yield ferulic acid, vanillic acid, p-coumaric acid and quercetin. TPC was 72.31 mg GAE/g of dried extract. CL treatment dose-dependently ameliorated gentamicin-induced impaired kidney and liver functions and improved the histopathological appearance of both organs. It also reduced gentamicin-induced oxidative stress. CL demonstrated downregulation of mRNA and protein expressions of IL-1 beta and NF-kappa B compared to nontreated rats. In silico interaction of the isolated compounds with amino acid residues of IL-1 beta and NF-kappa B might explain the current findings. Conclusion: Taken together, this study raises the waste-to-wealth potential of cauliflower to mitigate gentamicin-induced hepatorenal injury and convert the waste agromaterials into valuable products.

引用

页数：20