Oligonucleotide-stabilized fluorescent silver nanoclusters for turn-on detection of melamine

被引：78
|
作者：
Han, Shuang [1 ,2 ]
Zhu, Shuyun [1 ,2 ]
Liu, Zhongyuan [1 ]
Hu, Lianzhe [1 ,2 ]
Parveen, Saima [1 ,2 ,3 ]
Xu, Guobao [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
[3] Islamia Univ Bahawalpur, Dept Chem, Bahawalpur, Pakistan
来源：
BIOSENSORS & BIOELECTRONICS | 2012年 / 36卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Fluorescence; Oligonucleotide; Nanoclusters; Melamine; Silver; MASS-SPECTROMETRY; AG NANOCLUSTERS; KIDNEY TISSUE; FLUOROPHORES; CLUSTERS; SYSTEM; SENSOR;
D O I：
10.1016/j.bios.2012.04.028
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Fluorescence nanoclusters have been used for the determination of melamine for the first time. The method is based on the fluorescence turn-on of oligonucleotide-stabilized silver nanoclusters (DNA-Ag NCs) by melamine. The enhancement factors (I-I-0)/I-0 increase linearly with melamine concentrations over the range 5.0 x 10(-8)-7.0 x 10(-6) M (R-2=0.99. The detection limit is 1.0 x 10(-8) M, which is approximately 2000 times lower than the US Food and Drug Administration estimated melamine safety limit of 20.0 mu M. Furthermore, the milk samples spiked with melamine are analyzed with excellent recoveries. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：267 / 270
页数：4
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