Lysine Deprivation during Maternal Consumption of Low-Protein Diets Could Adversely Affect Early Embryo Development and Health in Adulthood

被引:7
作者
Van Winkle, Lon J. [1 ,2 ]
Galat, Vasiliy [3 ,4 ]
Iannaccone, Philip M. [5 ,6 ,7 ]
机构
[1] Midwestern Univ, Dept Biochem, Downers Grove, IL 60515 USA
[2] Rocky Vista Univ, Dept Med Humanities, 8401 S Chambers Rd, Parker, CO 80134 USA
[3] Northwestern Univ, Stanley Manne Childrens Res Inst, Dept Pathol, Feinberg Sch Med, Chicago, IL 60209 USA
[4] Ann & Robert H Lurie Childrens Hosp Chicago, Chicago, IL 60209 USA
[5] Northwestern Univ, Dept Pediat, Feinberg Sch Med, Chicago, IL 60209 USA
[6] Northwestern Univ, Dept Pathol, Feinberg Sch Med, Chicago, IL 60209 USA
[7] Lurie Childrens Hosp Chicago, Chicago, IL 60209 USA
关键词
barker hypothesis; blastocyst; embryo; embryonic stem cells; glutamate signaling; low-protein diet; lysine; offspring; AMINO-ACID-TRANSPORT; MOUSE EMBRYOS; STEM-CELLS; GLUTAMATE; DIFFERENTIATION; COMPETENCE; METABOLISM; BLASTOCYST; SUPPORTS;
D O I
10.3390/ijerph17155462
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The conversion of lysine to glutamate is needed for signaling in all plants and animals. In mouse embryonic stem (mES) cells, and probably their progenitors, endogenous glutamate production and signaling help maintain cellular pluripotency and proliferation, although the source of glutamate is yet to be determined. If the source of glutamate is lysine, then lysine deprivation caused by maternal low-protein diets could alter early embryo development and, consequently, the health of the offspring in adulthood. For these reasons, we measured three pertinent variables in human embryonic stem (hES) cells as a model for the inner cell masses of human blastocysts. We found that RNA encoding the alpha-aminoadipic semialdehyde synthase enzyme, which regulates glutamate production from lysine, was highly expressed in hES cells. Moreover, the mean amount of lysine consumed by hES cells was 50% greater than the mean amount of glutamate they produced, indicating that lysine is likely converted to glutamate in these cells. Finally, hES cells expressed RNA encoding at least two glutamate receptors. Since this may also be the case for hES progenitor cells in blastocysts, further studies are warranted to verify the presence of this signaling process in hES cells and to determine whether lysine deprivation alters early mammalian embryo development.
引用
收藏
页码:1 / 9
页数:9
相关论文
共 29 条
  • [1] Disruption of GRIN2B Impairs Differentiation in Human Neurons
    Bell, Scott
    Maussion, Gilles
    Jefri, Malvin
    Peng, Huashan
    Theroux, Jean-Francois
    Silveira, Heika
    Soubannier, Vincent
    Wu, Hanrong
    Hu, Peng
    Galat, Ekaterina
    Torres-Platas, S. Gabriela
    Boudreau-Pinsonneault, Camille
    O'Leary, Liam A.
    Galat, Vasiliy
    Turecki, Gustavo
    Durcan, Thomas M.
    Fon, Edward A.
    Mechawar, Naguib
    Ernst, Carl
    [J]. STEM CELL REPORTS, 2018, 11 (01): : 183 - 196
  • [2] Endogenous activation of mGlu5 metabotropic glutamate receptors supports self-renewal of cultured mouse embryonic stem cells
    Cappuccio, I
    Spinsanti, P
    Porcellini, A
    Desiderati, F
    De Vita, T
    Storto, M
    Capobianco, L
    Battaglia, G
    Nicoletti, F
    Melchiorri, D
    [J]. NEUROPHARMACOLOGY, 2005, 49 : 196 - 205
  • [3] New insights into human lysine degradation pathways with relevance to pyridoxine-dependent epilepsy due to antiquitin deficiency
    Crowther, Lisa M.
    Mathis, Deborah
    Poms, Martin
    Plecko, Barbara
    [J]. JOURNAL OF INHERITED METABOLIC DISEASE, 2019, 42 (04) : 620 - 628
  • [4] The Metabolomic Signature of Opa1 Deficiency in Rat Primary Cortical Neurons Shows Aspartate/Glutamate Depletion and Phospholipids Remodeling
    de la Barca, Juan Manuel Chao
    Arrazola, Macarena S.
    Bocca, Cinzia
    Arnaune-Pelloquin, Laetitia
    Iuliano, Olga
    Tcherkers, Guillaume
    Lenaers, Guy
    Simard, Gilles
    Belenguer, Pascale
    Reynier, Pascal
    [J]. SCIENTIFIC REPORTS, 2019, 9 (1)
  • [5] Metabolic Induction and Early Responses of Mouse Blastocyst Developmental Programming following Maternal Low Protein Diet Affecting Life-Long Health
    Eckert, Judith J.
    Porter, Richard
    Watkins, Adam J.
    Burt, Elizabeth
    Brooks, Suzanne
    Leese, Henry J.
    Humpherson, Peter G.
    Cameron, Iain T.
    Fleming, Tom P.
    [J]. PLOS ONE, 2012, 7 (12):
  • [6] Fleming TP, 2018, LANCET, V391, P1842, DOI [10.1016/S0140-6736(18)30312-X, 10.1016/s0140-6736(18)30312-x]
  • [7] Transgene Reactivation in Induced Pluripotent Stem Cell Derivatives and Reversion to Pluripotency of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
    Galat, Vasiliy
    Galat, Yekaterina
    Perepitchka, Mariana
    Jennings, Lawrence J.
    Iannaccone, Philip M.
    Hendrix, Mary J. C.
    [J]. STEM CELLS AND DEVELOPMENT, 2016, 25 (14) : 1060 - 1072
  • [8] A Model of Early Human Embryonic Stem Cell Differentiation Reveals Inter- and Intracellular Changes on Transition to Squamous Epithelium
    Galat, Vasiliy
    Malchenko, Sergey
    Galat, Yekaterina
    Ishkin, Alex
    Nikolsky, Yuri
    Kosak, Steven T.
    Soares, Bento Marcelo
    Iannaccone, Philip
    Crispino, John D.
    Hendrix, Mary J. C.
    [J]. STEM CELLS AND DEVELOPMENT, 2012, 21 (08) : 1250 - 1263
  • [9] Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential
    Galat, Yekaterina
    Dambaeva, Svetlana
    Elcheva, Irina
    Khanolkar, Aaruni
    Beaman, Kenneth
    Iannaccone, Philip M.
    Galat, Vasiliy
    [J]. STEM CELL RESEARCH & THERAPY, 2017, 8 : 1 - 11
  • [10] New insights into the regulation and functional significance of lysine metabolism in plants
    Galili, G
    [J]. ANNUAL REVIEW OF PLANT BIOLOGY, 2002, 53 : 27 - 43