High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

被引：10

作者：

Gabriel, Emma M. ^{[1
]}

Douarre, Pierre E. ^{[1
]}

Fitzgibbon, Siobhan ^{[2
]}

Clair, James ^{[2
]}

Lucey, Brigid ^{[1
,3
]}

Coffey, Aidan ^{[1
]}

O'Mahony, Jim M. ^{[1
]}

机构：

[1] Cork Inst Technol, Cork, Ireland

[2] Mercy Univ Hosp, Cork, Ireland

[3] Cork Univ Hosp, Cork, Ireland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 90卷 / 02期

关键词：

High resolution melting; Staphylococcus epidermidis; Linezolid resistance; G2576T mutation; MECHANISMS;

D O I：

10.1016/j.mimet.2012.04.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 235 rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay. (C) 2012 Elsevier B.V. All rights reserved.

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收藏

页码：134 / 136

页数：3

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