Studies on the control of Cylindrocladium buxicola using fungicides and host resistance

Cylindrocladium buxicola is a fungal pathogen that causes a severe leaf and twig blight oil Buxus spp. Laboratory experiments were carried Out to study the in vitro effect of 13 fungicides on mycelial growth and conidia germination of the fungus. Based on the values of the effective concentration at which mycelial growth was inhibited by 50%, the fungicide Stroby (kresoxim-methyl) and the combined fungicides Opponent (epoxiconazole + kresoxim-methyl + pyraclostrobin), Opera (epoxiconazole + pyraclostrobin), and Signum (boscalid + pyraclostrobin) were the most effective at inhibiting mycelial growth and conidia germination. Pathogenicity assays showed that the host range of the fungus was not limited to the genus Buxus because Sarcococca sp. also was Susceptible. None of the 10 boxwood species and cultivars tested were immune to the disease, although Buxus balearica as well as Sarcococca sp. showed significantly lower levels of infection as measured by the expression of leaf symptoms and the number of conidia produced on host tissue. the latter probably contributing to the apparent resistance of these species in the field. Microscopic observation showed that disease development was very rapid and aggressive on B. sempervirens 'Suffruticosa' where it was able to survive at least 5 years on decomposing fallen leaves.