Single-cell chromatin landscapes of mouse skin development

被引：1

作者：

Li, Fang ^{[1
]}

Xu, Tiantian ^{[1
]}

Li, Jiale ^{[1
]}

Hao, Xuxu ^{[1
]}

Ge, Wei ^{[2
]}

Wang, Xin ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

[2] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China

来源：

SCIENTIFIC DATA | 2022年 / 9卷 / 01期

基金：

中国国家自然科学基金;

关键词：

FOLLICLE; EXPRESSION; REVEALS; STATE; PROLIFERATION; MACROPHAGES; PROGENITORS; ACTIVATION; PRINCIPLES; INDUCTION;

D O I：

10.1038/s41597-022-01839-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The coat of mammals is produced by hair follicles, and hair follicle is an important and complex accessory organ of skin. As a complex physiological regulation process, hair follicle morphogenesis is regulated by a series of signal pathway factors, involves the interaction of multiple cell types and begins in the early embryonic stage. However, its transcriptional regulatory mechanism is unclear. We have therefore utilized single-cell ATAC sequencing to obtain the chromatin accessibility landscapes of 6,928, 6,961 and 7,374 high-quality cells from the dorsal skins of E13.5, E16.5 and P0 mice (Mus musculus), respectively. Based on marker gene activity clustering, we defined 6, 8 and 5 distinct cell types in E13.5, E16.5 and P0 stages, respectively. Furtherly, we integrated the fibroblasts and keratinocytes clusters, performed further analysis and re-clustered. The single cell map of the chromatin open area was drawn from each cell type and the mechanism of cell transcription regulation was explored. Collectively, our data provide a reference for deeply exploring the epigenetic regulation mechanism of mouse hair follicles development.

引用

页数：12

共 60 条

[1] Directional Cell Migration, but Not Proliferation, Drives Hair Placode Morphogenesis [J].