In vitro regeneration from immature embryos calli of durum wheat under salinity stress conditions

被引：0
|
作者：
Ayolie, Koutoua [1 ]
Patine, Mouhssine [1 ]
Houda, Elyacoubi [1 ]
Atmane, Rochdi [1 ]
机构：
[1] Ibn Tofail Univ, Fac Sci, Lab Nat Resources & Sustainable Dev, Kenitra, Morocco
来源：
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2020年 / 15卷 / 09期
关键词：
Durum wheat; immature embryos; callus; salt stress; in vitro regeneration; PLANT-REGENERATION; LEAF EXPLANTS; INDUCTION; CULTIVARS; RESPONSES; GENOTYPES; CULTURE; GROWTH;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Callus of eight durum wheat varieties (Amjad, Anouar, Marzak, Massa, Ourgh, Sebou, Tarek and Tomouh) was induced on immature embryos. Salt-selected calli and other non-selected were transferred to a first MS medium supplemented with 1 mg.l(-1) kinetin and 2 mg.l(-1) IAA and then to a second MS/2 medium supplemented with 1 mg.l(-1) kinetin only. Different concentrations of NaCl were added to each medium. The regeneration rate in both types of calli was reduced by sodium chloride and the reduction was very marked when the concentrations reached 12 and 16 g.l(-1) NaCl in culture medium. However, salt-selected calli reached a regeneration rate significantly higher than those induced without NaCl.
引用
收藏
页码：113 / 119
页数：7
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