Kif3a Guides Microtubular Dynamics, Migration and Lumen Formation of MDCK Cells

被引：24

作者：

Boehlke, Christopher ^{[1
]}

Kotsis, Fruzsina ^{[1
]}

Buchholz, Bjoern ^{[2
]}

Powelske, Christian ^{[1
]}

Eckardt, Kai-Uwe ^{[2
]}

Walz, Gerd ^{[1
,3
]}

Nitschke, Roland ^{[3
,4
]}

Kuehn, E. Wolfgang ^{[1
,3
]}

机构：

[1] Univ Hosp, Dept Nephrol, Freiburg, Germany

[2] Univ Hosp, Dept Nephrol, Erlangen, Germany

[3] Univ Freiburg, BIOSS Ctr Biol Signaling Studies, D-79106 Freiburg, Germany

[4] Univ Freiburg, Life Imaging Ctr, Ctr Biosyst Anal, D-79106 Freiburg, Germany

来源：

PLOS ONE | 2013年 / 8卷 / 05期

关键词：

INTRAFLAGELLAR TRANSPORT PROTEINS; ADENOMATOUS POLYPOSIS-COLI; KINESIN SUPERFAMILY; TIGHT JUNCTION; KIDNEY INJURY; PRIMARY CILIA; N-CADHERIN; POLARITY; GROWTH; ENDS;

D O I：

10.1371/journal.pone.0062165

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The microtubular motor Kinesin-2 and its subunit Kif3a are essential for the formation of primary cilia, an organelle implicated in a wide spectrum of developmental abnormalities. Outside cilia, Kinesin-2 mediated transport has been implicated in vesicle and N-cadherin transport, but it is unknown if and how extraciliary Kif3a affects basic cellular functions such as migration or the formation of multicellular structures. Here we show that tetracycline inducible depletion of Kif3a in MDCK cells slows epithelial cell migration. Microtubules at the leading edge of Kif3a depleted cells failed to grow perpendicularly into the leading edge and microtubular dynamics were dampened in Kif3a depleted cells. Loss of Kif3a retarded lateral membrane specification and completely prevented the formation of three-dimensional spheres in collagen. These data uncover that Kif3a regulates the microtubular cytoskeleton in the cell periphery and imply that extra-ciliary Kif3a has an unexpected function in morphogenesis.

引用

页数：12

共 45 条

[31] Shaping microtubules into diverse patterns: Molecular connections for setting up both ends [J].