Molecular identification of Anopheles (Cellia) sundaicus from the Andaman and Nicobar islands of India

被引:24
作者
Alam, MT
Das, MK
Ansari, MA
Sharma, YD
机构
[1] All India Inst Med Sci, Dept Biotechnol, New Delhi 110029, India
[2] Malaria Res Ctr, Field Stn, Andaman Nicobar 744301, India
[3] Malaria Res Ctr, Delhi 110054, India
关键词
An; sundaicus; malaria vector; sibling species; ribosomal DNA sequence;
D O I
10.1016/j.actatropica.2005.07.028
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Anopheles (Cellia) sundaicus (Rodenwaldt) is an important malaria vector in the Andaman and Nicobar islands of India where it breeds in freshwater as well as in brackish water. To establish the molecular identity of An. sundaicus on these islands we analyzed samples from four geographically isolated areas-Teressa, Nancowry, Car Nicobar and Katchal islands. PCR-amplification and nucleotide sequence analysis were performed for internal transcribed spacer 2 (ITS2) and domain-3 (D3) of 28S rRNA. The ITS2 region of An. sundaicus from all four islands was identical but different from An. sundaicus A of Vietnam and An. sundaicus s.s of Malaysia. Furthermore, freshwater and brackish water forms of An. sundaicus did not reveal any sequence variation. Similarly, the D3 sequences were identical among all An. sundaicus samples from the four islands. D3 sequences for a species of the Sundaicus Complex are reported here for the first time and thus could not be compared with other regional isolates of this species. In conclusion, probably only one member of the Sundaicus Complex exists on the Andaman and Nicobar islands, which breeds in freshwater as well as in brackish water and is different from the An. sundaicus A and Malaysian An. sundaicus s.s. The identification of a new sibling species of the Sundaicus Complex in these islands is significant from the viewpoint of vector control strategies. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:10 / 18
页数:9
相关论文
共 40 条
  • [1] Comparative susceptibility of three important malaria vectors Anopheles stephensi, Anopheles fluviatilis and Anopheles sundaicus to Plasmodium vivax
    Adak, T
    Singh, OP
    Das, MK
    Wattal, S
    Nanda, N
    [J]. JOURNAL OF PARASITOLOGY, 2005, 91 (01) : 79 - 82
  • [2] Alvarez JM, 2002, ANN ENTOMOL SOC AM, V95, P250, DOI 10.1603/0013-8746(2002)095[0250:EOTRID]2.0.CO
  • [3] 2
  • [4] BRANDLING-BENNETT A D, 1981, Southeast Asian Journal of Tropical Medicine and Public Health, V12, P289
  • [5] CHANG MS, 2001, MEKONG MALARIA FORUM, V8, P91
  • [6] Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species
    Cohuet, A
    Simard, F
    Toto, JC
    Kengne, P
    Coetzee, M
    Fontenille, D
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 69 (02) : 200 - 205
  • [7] A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species
    Collins, FH
    Paskewitz, SM
    [J]. INSECT MOLECULAR BIOLOGY, 1996, 5 (01) : 1 - 9
  • [8] Collins RT, 1979, 79 WHOMAL
  • [9] Coluzzi M., 1967, Parassitologia, V9, P73
  • [10] Das M. K., 1998, Indian Journal of Malariology, V35, P197