Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging

被引：112

作者：

Zang, Xinshan ^{[1
]}

Geng, Xiaoli ^{[1
]}

Wang, Fei ^{[1
]}

Liu, Zhenshan ^{[1
]}

Zhang, Liyuan ^{[1
]}

Zhao, Yue ^{[1
]}

Tian, Xuejun ^{[1
]}

Ni, Zhongfu ^{[1
]}

Yao, Yingyin ^{[1
]}

Xin, Mingming ^{[1
]}

Hu, Zhaorong ^{[1
]}

Sun, Qixin ^{[1
]}

Peng, Huiru ^{[1
]}

机构：

[1] China Agr Univ, Beijing Key Lab Crop Genet Improvement, State Key Lab Agrobiotechnol, Key Lab Crop Heterosis & Utilizat MOE, 2 Yuanmingyuan Xi Rd, Beijing 100193, Peoples R China

来源：

BMC PLANT BIOLOGY | 2017年 / 17卷

基金：

中国国家自然科学基金;

关键词：

TaFER-5B; Heat stress; Abiotic stress; Ferritin-lacking mutant; Wheat; Arabidopsis; DIFFERENTIAL EXPRESSION; TRANSCRIPTOME ANALYSIS; EXTENSION PEPTIDE; PLANT FERRITIN; SEED FERRITIN; IRON; ARABIDOPSIS; RESPONSES; ANIMALS; PROTEIN;

D O I：

10.1186/s12870-016-0958-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: The yield of wheat (Triticum aestivum L.), an important crop, is adversely affected by heat stress in many regions of the world. However, the molecular mechanisms underlying thermotolerance are largely unknown. Results: A novel ferritin gene, TaFER, was identified from our previous heat stress-responsive transcriptome analysis of a heat-tolerant wheat cultivar (TAM107). TaFER was mapped to chromosome 5B and named TaFER-5B. Expression pattern analysis revealed that TaFER-5B was induced by heat, polyethylene glycol (PEG), H2O2 and Feethylenediaminedi(o-hydroxyphenylacetic) acid (Fe-EDDHA). To confirm the function of TaFER-5B in wheat, TaFER-5B was transformed into the wheat cultivar Jimai5265 (JM5265), and the transgenic plants exhibited enhanced thermotolerance. To examine whether the function of ferritin from mono-and dico-species is conserved, TaFER-5B was transformed into Arabidopsis, and overexpression of TaFER-5B functionally complemented the heat stresssensitive phenotype of a ferritin-lacking mutant of Arabidopsis. Moreover, TaFER-5B is essential for protecting cells against heat stress associated with protecting cells against ROS. In addition, TaFER-5B overexpression also enhanced drought, oxidative and excess iron stress tolerance associated with the ROS scavenging. Finally, TaFER-5B transgenic Arabidopsis and wheat plants exhibited improved leaf iron content. Conclusions: Our results suggest that TaFER-5B plays an important role in enhancing tolerance to heat stress and other abiotic stresses associated with the ROS scavenging.

引用

页数：13

共 42 条

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