GENETIC DIVERSITY IN BRASILIAN AMAZON COLLECTIONS OF CUPUASSU TREE GERMPLASM [Theobroma grandiflorum (Willd. ex Spreng.) Schum]

被引:12
作者
Alves, Rafael Moyses [1 ]
de Sousa Silva, Carlos Rogerio [2 ]
da Conceicao Silva, Maria Suellem [3 ]
de Souza Silva, Darly Caroline
Sebbenn, Alexandre Magno [4 ]
机构
[1] Embrapa Amazonia Oriental, Belem, Para, Brazil
[2] CEPLAC, ERJOH, Marituba, PA, Brazil
[3] Univ Estadual Paulista, Ilha Solteira, SP, Brazil
[4] Inst Florestal Sao Paulo, Sao Paulo, Brazil
关键词
Microsatellite; inbreeding; genetic diversity; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; LINKAGE MAP; CACAO L; PROGRAM;
D O I
10.1590/S0100-29452013000300019
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
This study aimed to evaluate the genetic diversity and relatedness of 24 accessions of Theobroma grandiflorum, originating from three units of Embrapa, aiming their use as parents in hybridization specie programs. The genetic markers used were heterologous microsatellite loci developed for cocoa. In the population studied 45 alleles were found. The effective average number of alleles per locus (2.33) was less than the average number of alleles per locus (3.21), indicating that many alleles have low frequency. The observed heterozygosity at polymorphic loci ranged from 0.33 to 1.00 with a mean of 0.54 and expected heterozygosity ranged from 0.48 to 0.76 with a mean of 0.54. The fixation index medium between loci (0.003) was not significantly different from zero. The estimate of relatedness between pairs of individuals indicates that some may be relatives, including half-brothers and clones. The results suggest that the accesses of T. grandiflorum analyzed contain a moderate level of genetic diversity and absence of inbreeding and therefore great potential for use in breeding programs.
引用
收藏
页码:818 / 828
页数:11
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