Characterization of the expression of Na+, K+-ATPase subunits in human breast cancer cells

被引：0

作者：

Dias M., Almeida ^{[1
]}

Capella M. A., Marques ^{[1
]}

Lopes, A. G. ^{[1
]}

机构：

[1] Inst Biofis Carlos Chagas Filho Brazil, Rio De Janeiro, Brazil

来源：

ICCB 2012: PROCEEDINGS OF THE 10TH INTERNATIONAL CONGRESS ON CELL BIOLOGY | 2013年

关键词：

Na+; K+-ATPase; breast cancer; MCF7; MDA-MB-231; TIGHT JUNCTIONS; E-CADHERIN; NA; K-ATPASE; ADHERENS; OUABAIN;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: It is well established that the known Na+, K+-ATPase has cell adhesion molecule role in adherens junctions [16]. In so, beta subunit acts on maturation and membrane targeting of a catalytic subunit. Studies have demonstrated the importance of the sodium permeability in tumorigenesis, but there's no study of Na+, K+-ATPase alpha and beta subunit's expression. The aim of the present study is to characterize the expression of Na+, K+-ATPase subunits in human breast cancer cells. Methods and Results: To observe the expression of alpha 1 and beta 1 in MCF-7, MDA-231 and MDCK cells were seeded in 6 well plates for 24, 48 and 72 hours. The measurement of of alpha 1 and beta 1 expression by Western Blotting showed that tumor cell lines do not express beta 1 subunit. Conclusion: Our results indicate that there's a distinct adhesion mechanism in tumor cells, which should explain metastasis.

引用

页码：7 / 10

页数：4

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