The Interaction of Ferric Ions with Jack Bean Urease by Isothermal Titration Calorimetry

被引：2
|
作者：
Behbehani, G. Rezaei [1 ]
Barzegar, L. [1 ]
机构：
[1] Islamic Azad Univ, Takestan Branch, Dept Chem, Fac Sci, Takestan, Iran
来源：
NATIONAL ACADEMY SCIENCE LETTERS-INDIA | 2013年 / 36卷 / 04期
关键词：
Isothermal titration calorimetry; Jack bean urease; Fe3+ ion; Binding parameters; N-(N-BUTYL) THIOPHOSPHORIC TRIAMIDE; NICKEL ION; BINDING;
D O I：
10.1007/s40009-013-0145-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of the investigations was to measure the influence of Fe3+ ion on jack bean urease (JBU) activity. Interaction between Fe3+ and JBU, is examined using isothermal titration calorimetry. It was found that Fe3+ ions acted as a non-cooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for Fe3+ ions. The small structural parameters show that there are little changes on the JBU structure, indicating that Fe3+ has minor effect on the JBU activity. The association equilibrium constant is 42,484.13 +/- A 110 mol l(-1), indicating the moderate interaction of Fe3+ ion with JBU. The molar enthalpy of binding is Delta H = -4.33 kJ mol(-1).
引用
收藏
页码：393 / 395
页数：3
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