MicroRNA-155 is a novel suppressor of ovarian cancer-initiating cells that targets CLDN1

被引：126
作者：
Qin, Wenxing [1 ]
Ren, Qiusheng [4 ]
Liu, Te [1 ,2 ]
Huang, Yongyi [3 ]
Wang, Jiejun [1 ]
机构：
[1] Second Mil Med Univ, Dept Med Oncol, Shanghai Changzheng Hosp, Shanghai 200070, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Shanghai Geriatr Inst Chinese Med, Shanghai 200031, Peoples R China
[3] Univ Sud Toulon Var, Lab PROTEE, F-83957 La Garde, France
[4] Shanghai Jiading Hosp Chinese Med, Shanghai, Peoples R China
来源：
FEBS LETTERS | 2013年 / 587卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Ovarian cancer-initiating cell (OCIC); MicroRNA-155; CLDN1; Invasion; TYPE-1 RECEPTOR EXPRESSION; BREAST-CANCER; UNTRANSLATED REGION; ENDOTHELIAL-CELLS; CLAUDIN-1; MIR-155; RNA;
D O I：
10.1016/j.febslet.2013.03.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Previous cDNA microarrays indicated that CLDN1 (claudin-1) is an important gene for ovarian cancer-initiating cell (OCIC) invasion and adhesion. Here, we show that the downregulation of miR-155 in OCICs correlates with CLDN1 overexpression and the suppression of OCIC invasion. Luciferase assays indicate that miR-155 targets CLDN1 mRNA on the 3' UTR. CLDN1 mRNA and claudin-1 protein expression were significantly decreased in miR-155-OCICs. Proliferation assays and Transwell migration assays show that miR-155 significantly suppresses the proliferative and invasive capacity of OCICs. Furthermore, miR-155 suppresses the growth of OCIC xenograft tumors. Thus, overexpression of miR-155 may prevent tumorigenesis in human ovarian cancer through downregulation of CLDN1. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：1434 / 1439
页数：6
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