Kinetics of antibody-secreting cell and fecal IgA responses after oral cholera vaccination in different age groups in a cholera endemic country

被引：17

作者：

Akhtar, Marjahan ^{[1
]}

Qadri, Firdausi ^{[1
]}

Bhuiyan, Taufiqur R. ^{[1
]}

Akter, Sarmin ^{[1
]}

Rafique, Tanzeem A. ^{[1
]}

Khan, Arifuzzaman ^{[1
]}

Islam, Laila N. ^{[2
]}

Saha, Amit ^{[1
]}

Svennerholm, Ann-Mari ^{[3
]}

Lundgren, Anna ^{[3
]}

机构：

[1] Icddr B, Dhaka, Bangladesh

[2] Univ Dhaka, Dept Biochem & Mol Biol, Dhaka, Bangladesh

[3] Univ Gothenburg, Inst Biomed, Dept Microbiol & Immunol, GUVAX Gothenburg Univ Vaccine Res Inst, Gothenburg, Sweden

来源：

VACCINE | 2017年 / 35卷 / 02期

基金：

瑞典研究理事会;

关键词：

Antibody-secreting cell; Antibodies in lymphocyte supernatant; IgA; Oral vaccine; Cholera; Children; ENTEROTOXIGENIC ESCHERICHIA-COLI; B-SUBUNIT VACCINE; INTESTINAL IMMUNE-RESPONSES; PERIPHERAL-BLOOD; TYPHOID VACCINE; DOUBLE-BLIND; IMMUNOGENICITY; SAFETY; IMMUNIZATION; CHILDREN;

D O I：

10.1016/j.vaccine.2016.11.055

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immune responses to oral enteric vaccines in children and infants may be influenced by factors such as age, previous priming with related microorganisms and breast feeding. In this study, we aimed to determine optimal time points to assess immune responses to oral enteric vaccines in different clinical specimens. This was done by investigating antibody secreting cell (ASC) and fecal antibody responses on different days after vaccination using the licensed oral cholera vaccine Dukoral, containing cholera toxin B-subunit (rCTB) and inactivated Vibrio cholerae bacteria, as a model vaccine. Two vaccine doses were given 2 weeks apart to infants (6-11 months), young children (12-18 months), toddlers (19 months-5 years) and adults in a cholera endemic country (Bangladesh). IgA ASC responses, as determined by the antibodies in lymphocyte supernatant (ALS) assay, plasma IgA and IgG responses and secretory IgA (SIgA) responses in extracts of fecal samples were evaluated 4/5 and 7 days after each vaccination. After the first vaccine dose, anti-CTB ALS IgA responses in adults and toddlers were high and comparable on day 5 and 7, while responses were low and infrequent in young children. After the second dose, highest ALS responses were detected on day 5 among the time points studied in all age groups and the responses declined until day 7. In contrast, plasma IgA and IgG anti-CTB responses were high both on day 5 and 7 after the second dose. Fecal SIgA responses in young children and infants were highest on day 7 after the second dose. Our results suggest that ASC/ALS responses to two doses of the oral cholera vaccine Dukoral and related oral vaccines should be analyzed earlier than previously recommended (day 7) at all ages. Fecal antibody responses should preferably be analyzed later than ASC/ALS responses to detect the highest antibody responses. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：321 / 328

页数：8

共 34 条

[1]

Åhrén C, 1998, INFECT IMMUN, V66, P3311

[2]

[Anonymous], 2010, WHO GLOBAL DATA BANK

[3] Evaluation of immune responses to an oral typhoid vaccine, Ty21a, in children from 2 to 5 years of age in Bangladesh [J].