Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence

被引：40

作者：

Jara-Guajardo, Pedro ^{[1
,2
]}

Cabrera, Pablo ^{[1
,2
]}

Celis, Freddy ^{[3
]}

Soler, Monica ^{[4
]}

Berlanga, Isadora ^{[4
]}

Parra-Munoz, Nicole ^{[4
]}

Acosta, Gerardo ^{[5
,6
]}

Albericio, Fernando ^{[5
,6
,7
]}

Guzman, Fanny ^{[8
]}

Campos, Marcelo ^{[9
]}

Alvarez, Alejandra ^{[10
,11
]}

Morales-Zavala, Francisco ^{[1
,2
]}

Kogan, Marcelo J. ^{[1
,2
]}

机构：

[1] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Quim Farmacol & Toxicol, Santiago 8380494, Chile

[2] Adv Ctr Chron Dis ACCDiS, Sergio Livingstone 1007, Santiago 8380494, Chile

[3] Univ Playa Ancha, Lab Proc Foton & Electroquim, Playa Ancha 850, Valparaiso, Chile

[4] Univ Chile, Fac Ciencias Fis & Matemat, Dept Ingn Quim Biotecnol & Mat, Santiago 8380494, Chile

[5] Univ Barcelona UB, CIBER BBN, Networking Ctr Bioengn Biomat & Nanomed, Marti i Franques 1-11, Barcelona 08028, Spain

[6] Univ Barcelona UB, Dept Organ Chem, Marti i Franques 1-11, Barcelona 08028, Spain

[7] Univ KwaZulu Natal, Sch Chem & Phys, ZA-4001 Durban, South Africa

[8] Pontificia Univ Catolica Valparaiso, Nucleo Biotecnol Curauma NBC, Valparaiso 2460355, Chile

[9] Univ Chile, Dept Chem, Fac Sci, POB 653, Santiago 8380494, Chile

[10] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Alameda 340, Santiago 8331010, Chile

[11] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Envejecimiento & Regenerac CARE, Santiago 8380494, Chile

来源：

NANOMATERIALS | 2020年 / 10卷 / 04期

关键词：

gold nanorods; CRANAD-2; amyloid beta peptide; SEF; Alzheimer's disease; ALZHEIMERS-DISEASE; PEPTIDE; NANORODS; RAMAN; CYTOTOXICITY; STRATEGIES;

D O I：

10.3390/nano10040690

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The early detection of the amyloid beta peptide aggregates involved in Alzheimer's disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer's disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo.

引用

页数：16

共 35 条

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