Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

被引：355

作者：

Backman, V

Gurjar, R

Badizadegan, K

Itzkan, L

Dasari, RR

Perelman, LT

Feld, MS

机构：

[1] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA

[2] Childrens Hosp, Dept Pathol, Boston, MA 02115 USA

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 1999年 / 5卷 / 04期

基金：

美国国家卫生研究院;

关键词：

cancer diagnosis; light scattering; medical spectroscopy; polarization;

D O I：

10.1109/2944.796325

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.

引用

页码：1019 / 1026

页数：8

共 21 条

[1] POLARIZED-LIGHT EXAMINATION AND PHOTOGRAPHY OF THE SKIN [J].