Simple device for the direct visualization of oral-cavity tissue fluorescence

被引:258
作者
Lane, PM
Gilhuly, T
Whitehead, P
Zeng, HS
Poh, CF
Ng, S
Williams, PM
Zhang, LW
Rosin, MP
MacAulay, CE
机构
[1] British Columbia Canc Res Ctr, Canc Imaging Dept, Vancouver, BC V5Z 1L3, Canada
[2] LED Med Diagnost Inc, White Rock, BC V4B 1C5, Canada
[3] Univ British Columbia, Fac Dent, Vancouver, BC V6T 1Z4, Canada
[4] British Columbia Canc Res Ctr, Oral Oncol Dept, Vancouver, BC V5Z 1L3, Canada
[5] British Columbia Canc Res Ctr, Canc Control Res Program, Vancouver, BC V5Z 1L3, Canada
[6] Simon Fraser Univ, Burnaby, BC V5A 1S6, Canada
关键词
autofluorescence; fluorescence imaging; oral cancer; squamous cell carcinoma;
D O I
10.1117/1.2193157
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Early identification of high-risk disease could greatly reduce both mortality and morbidity due to oral cancer. We describe a simple handheld device that facilitates the direct visualization of oral-cavity fluorescence for the detection of high-risk precancerous and early cancerous lesions. Blue excitation light (400 to 460 nm) is employed to excite green-red fluorescence from fluorophores in the oral tissues. Tissue fluorescence is viewed directly along an optical axis collinear with the axis of excitation to reduce inter- and intraoperator variability. This robust, field-of-view device enables the direct visualization of fluorescence in the context of surrounding normal tissue. Results from a pilot study of 44 patients are presented. Using histology as the gold standard, the device achieves a sensitivity of 98% and specificity of 100% when discriminating normal mucosa from severe dysplasia/carcinoma in situ (CIS) or invasive carcinoma. We envisage this device as a suitable adjunct for oral cancer screening, biopsy guidance, and margin delineation. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
引用
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页数:7
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