Effects of sound field on the growth of Chrysanthemum callus

The effects of sound field on Chrysanthemum callus were studied. The field of alternative stress was generated through a strong sound field system set up in our lab, and the activity of SOD, the content of soluble proteins, the activity of IAA oxidase and the absorption rate of calcium were measured. We found that the sound field stimulation has dual effects, which can enhance or inhibit the growth of Chrysanthemum callus, the growth effects of sound field on Chrysanthemum callus depended greatly on the intensity and frequency of sound field. The activity of SOD, the content of soluble proteins and the absorption rate of calcium in callus increased with the intensity and frequency increasing. However, those indexes began to decrease when the intensity and frequency went beyond the limit of 100 dB and 800 Hz, respectively. However, the changing tendency of lAA oxidase activity was reversed to the above three indexes. We draw a conclusion that the optimal stimulation conditions are 100 dB and 800 Hz. Under the conditions, the sound field can distinctly enhance the growth of Chrysanthemum callus. We think that moderate stress stimulation can enhance the assimilation of tissues or cells, improve their physiological activity and accelerate the growth of plants, this moderate stress stimulation is helpful to the growth of plant tissues. (C) 2002 Elsevier Science B.V. All rights reserved.