A Novel Antimicrobial Peptide from Skin Secretionsof the Tree Frog Theloderma kwangsiensis

被引：4

作者：

Yan, Hongli ^{[1
]}

Liu, Yingying ^{[3
]}

Tang, Jing ^{[1
]}

Mo, Guoxiang ^{[1
]}

Song, Yuzhu ^{[2
]}

Yan, Xiuwen ^{[1
]}

Wei, Lin ^{[1
]}

Lai, Ren ^{[1
]}

机构：

[1] Nanjing Agr Univ, Life Sci Coll, Nanjing 210095, Jiangsu, Peoples R China

[2] Kunming Univ Sci & Technol, Life Sci & Technol Coll, Kunming 650093, Yunnan, Peoples R China

[3] 3 People Hosp Yunnan Prov, Dept Endocrinol, Kunming 650011, Peoples R China

来源：

ZOOLOGICAL SCIENCE | 2013年 / 30卷 / 09期

关键词：

Amphibia; antimicrobial peptide; tree frog; skin secretion; Theloderma kwangsiensis; FAMILIES; SEQUENCE;

D O I：

10.2108/zsj.30.704

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Most of amphibians belonging to family Rhacophoridae live in arboreal habitats. A large number of antimicrobial peptides (AMPs) have been identified from amphibian skins. No antimicrobial peptide from Rhacophoridae amphibians has been reported. In this study, we purified and characterized a novel antimicrobial peptide, pleurain-a1-thel from skin secretions of the tree frog, Theloderma kwangsiensis. Its amino acid sequence was determined as RILTMTKRVKMPQLYKQIVCRLFKTC by Edman degradation, mass spectrometry analysis and cDNA cloning. There are two cysteines, which form an intra-molecular disulfide bridge, in the sequence of pleurain-a1-thel. Pleurain-a1-thel exerted potential antimicrobial activities against wide spectrum of microorganisms, including Gram-negative and -positive bacteria and fungi. It exerted little hemolytic activity in human or rabbit red cells. To the best of our knowledge, this is the first report of antimicrobial peptide from Rhacophoridae amphibians.

引用

页码：704 / 709

页数：6

共 32 条

[1] AMPHIBIAN SKIN - A PROMISING RESOURCE FOR ANTIMICROBIAL PEPTIDES [J].

BARRA, D ;

SIMMACO, M .

TRENDS IN BIOTECHNOLOGY, 1995, 13 (06) :205-209

[2]

BEVINS CL, 1990, ANNU REV BIOCHEM, V59, P395, DOI 10.1146/annurev.biochem.59.1.395

[3] A RAPID AND SENSITIVE HEMOLYSIS NEUTRALIZATION ASSAY FOR PALYTOXIN [J].

BIGNAMI, GS .

TOXICON, 1993, 31 (06) :817-820

[4] ANTIBACTERIAL PEPTIDES - KEY COMPONENTS NEEDED IN IMMUNITY [J].

BOMAN, HG .

CELL, 1991, 65 (02) :205-207

[5] The Chinese bamboo leaf odorous frog (Rana (Odorrana) versabilis) and North American Rana frogs share the same families of skin antimicrobial peptides [J].

Chen, Tianbao ;

Zhou, Mei ;

Rao, Pingfan ;

Walker, Brian ;

Shaw, Chris .

PEPTIDES, 2006, 27 (07) :1738-1744

[6] Antimicrobial peptides from ranid frogs: taxonomic and phylogenetic markers and a potential source of new therapeutic agents [J].

Conlon, JM ;

Kolodziejek, J ;

Nowotny, N .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2004, 1696 (01) :1-14

[7]

Dubois Alain, 1992, Bulletin Mensuel de la Societe Linneenne de Lyon, V61, P305

[8]

FROST D.R., 2011, Amphibian Species of the World: an online referencet

[9] Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial [J].

He, Weiyu ;

Feng, Feifei ;

Huang, Yong ;

Guo, Huanhuan ;

Zhang, Songyan ;

Li, Zheng ;

Liu, Jingze ;

Wang, Yipeng ;

Yu, Haining .

BIOCHIMIE, 2012, 94 (03) :649-655

[10] A protein with antimicrobial activity in the skin of Schlegel's green tree frog Rhacophorus schlegelii (Rhacophoridae) identified as histone H2B [J].

Kawasaki, H ;

Isaacson, T ;

Iwamuro, S ;

Conlon, JM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 312 (04) :1082-1086

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