Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

被引：9
作者：
Adhikari, Khem Bahadur [1 ]
Rohde, Morten [2 ]
Velschow, Sten [2 ]
Feldt-Rasmussen, Ulla [3 ,4 ]
Johannesen, Jesper [4 ,5 ]
Johnsen, Anders Holten [1 ]
机构：
[1] Rigshosp, Dept Clin Biochem, KB3011, Copenhagen, Denmark
[2] Fluisense ApS, Lillerod, Denmark
[3] Rigshosp, Dept Endocrinol & Metab, Copenhagen, Denmark
[4] Univ Copenhagen, Fac Hlth & Med Sci, Copenhagen, Denmark
[5] Herlev & Gentofte Hosp, Dept Pediat, Copenhagen, Denmark
来源：
BIOANALYSIS | 2020年 / 12卷 / 10期
关键词：
cortisol; dried blood spot; fluispotter; hematocrit; LC-MS/MS; microsampling; CONGENITAL ADRENAL-HYPERPLASIA; TANDEM MASS-SPECTROMETRY; QUANTITATIVE BIOANALYSIS; EBF RECOMMENDATION; DBS; HEMATOCRIT; QUANTIFICATION; VALIDATION; COLLECTION; MS/MS;
D O I：
10.4155/bio-2020-0048
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 mu l DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.
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收藏
页码：665 / 681
页数：17
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