Quantitative expression analysis of the apoptosis-related gene, BCL2L12, in head and neck squamous cell carcinoma

被引:20
作者
Geomela, Panagiota-Aikaterini [1 ]
Kontos, Christos K. [1 ]
Yiotakis, Ioannis [2 ,3 ,4 ]
Scorilas, Andreas [1 ]
机构
[1] Univ Athens, Fac Biol, Dept Biochem & Mol Biol, Athens 15701, Greece
[2] Univ Athens, Athens Gen Hosp Hippokrat, Fac Med, Ear Clin 1, Athens 15701, Greece
[3] Univ Athens, Athens Gen Hosp Hippokrat, Fac Med, Nose Clin, Athens 15701, Greece
[4] Univ Athens, Athens Gen Hosp Hippokrat, Fac Med, Throat Clin, Athens 15701, Greece
基金
欧盟第七框架计划;
关键词
BCL2; family; larynx; oral cancer; tongue; tumor biomarkers; GROWTH-FACTOR RECEPTOR; CYCLIN D1 GENE; PROGNOSTIC-SIGNIFICANCE; BCL-2; EXPRESSION; POOR-PROGNOSIS; MESSENGER-RNA; P53; MARKERS; CANCER; MEMBER;
D O I
10.1111/j.1600-0714.2012.01190.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: BCL2L12 is a recently identified gene belonging to the BCL2 family, members of which are implicated in head and neck squamous cell carcinoma (HNSCC). We have recently shown that BCL2L12 mRNA expression is an unfavorable prognostic indicator in nasopharyngeal carcinoma (NPC) and that BCL2L12 can be regarded as a novel, useful tissue biomarker for the prediction of NPC patients' short-term relapse. The aim of this study was to analyze the mRNA expression of the novel apoptosis-related gene BCL2L12 in patients with HNSCC. Methods: Total RNA was isolated from 53 malignant tumors originating in larynx, pharynx, tongue, buccal mucosa, parotid glands, and nasal cavity, as well as from 34 adjacent non-cancerous tissue specimens, resected from patients with HNSCC. A highly sensitive real-time PCR method for BCL2L12 mRNA quantification in head and neck tissues was developed using the SYBR (R) Green chemistry. After preparing cDNA by reverse transcription, relative quantification was performed using the comparative CT () method. Results: BCL2L12 mRNA levels were lower in laryngeal tumors of advanced tumor, node, metastasis (TNM) stage or bigger size and in well-differentiated malignant tongue neoplasms, compared with early-stage laryngeal tumors or poorly differentiated tongue tumors. Interestingly, the BCL2L12 expression showed significant discriminatory value, distinguishing efficiently patients with tongue squamous cell carcinoma (SCC) from non-cancerous population. Conclusions: This is the first study examining the BCL2L12 mRNA expression in HNSCC. Our results suggest that BCL2L12 mRNA expression may serve as a potential prognostic biomarker in tongue and/or larynx SCC, which principally constitute the great majority of HNSCC cases worldwide. J Oral Pathol Med (2013)42: 154-161
引用
收藏
页码:154 / 161
页数:8
相关论文
共 59 条
[1]   Head and neck cancer [J].
Argiris, Athanassios ;
Karamouzis, Michalis V. ;
Raben, David ;
Ferris, Robert L. .
LANCET, 2008, 371 (9625) :1695-1709
[2]  
Assimakopoulos D, 2000, ANTICANCER RES, V20, P3555
[3]   Prognostic significance of p16INK4a alterations and 9p21 loss of heterozigosity in locally advanced laryngeal squamous cell carcinoma [J].
Bazan, V ;
Zanna, I ;
Migliavacca, M ;
Sanz-Casla, MT ;
Maestro, ML ;
Corsale, S ;
Macaluso, M ;
Dardanoni, G ;
Restivo, S ;
Quintela, PL ;
Bernaldez, R ;
Salerno, S ;
Morello, V ;
Tomasino, RM ;
Gebbia, N ;
Russo, A .
JOURNAL OF CELLULAR PHYSIOLOGY, 2002, 192 (03) :286-293
[4]  
Bellacosa A, 1996, CLIN CANCER RES, V2, P175
[5]   New technologies for studying the complexity of oral diseases [J].
Burbelo, P. D. ;
Bayat, A. ;
Lebovitz, E. E. ;
Iadarola, M. J. .
ORAL DISEASES, 2012, 18 (02) :121-126
[6]   Prognostic impact of bcl-2 expression on advanced nasopharyngeal carcinoma [J].
Chen, Mu-Kuan ;
Lai, Jui-Chung ;
Chang, Cheng-Chuan ;
Chang, Julia H. ;
Chang, Yu-Jun ;
Chen, Hui-Chuan .
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2008, 30 (08) :1052-1057
[7]   Expression of bcl-2 correlates with poor prognosis and modulates migration of nasopharyngeal carcinoma cells [J].
Chen, Mu-Kuan ;
Yang, Shun-Fa ;
Lai, Jui-Chung ;
Yeh, Kun-Tu ;
Yang, Jia-Sin ;
Chen, Li-Sheng ;
Chen, Hui-Chuan .
CLINICA CHIMICA ACTA, 2010, 411 (5-6) :400-405
[8]   Head and neck cancer: past, present and future [J].
Chin, David ;
Boyle, Glen M. ;
Porceddu, Sandro ;
Theile, David R. ;
Parsons, Peter G. ;
Coman, William B. .
EXPERT REVIEW OF ANTICANCER THERAPY, 2006, 6 (07) :1111-1118
[9]   Increased epidermal growth factor receptor gene copy number is associated with poor prognosis in head and neck squamous cell carcinomas [J].
Chung, Christine H. ;
Ely, Kim ;
McGavran, Loris ;
Varella-Garcia, Marileila ;
Parker, Joel ;
Parker, Natalie ;
Jarrett, Carolyn ;
Carter, Jesse ;
Murphy, Barbara A. ;
Netterville, James ;
Burkey, Brian B. ;
Sinard, Robert ;
Cmelak, Anthony ;
Levy, Shawn ;
Yarbrough, Wendell G. ;
Slebos, Robbert J. C. ;
Hirsch, Fred R. .
JOURNAL OF CLINICAL ONCOLOGY, 2006, 24 (25) :4170-4176
[10]  
de Vicente JC, 2006, J ORAL PATHOL MED, V35, P140