Phloroglucinol Mediated Shoot Bud Elongation in Capsicum annuum L.

被引：8

作者：

Bairwa, Vinod Kumar ^{[1
]}

Kachhwaha, Sumita ^{[1
]}

Kothari, S. L. ^{[1
]}

机构：

[1] Univ Rajasthan, Dept Bot, Jaipur 302004, Rajasthan, India

来源：

NATIONAL ACADEMY SCIENCE LETTERS-INDIA | 2012年 / 35卷 / 04期

关键词：

Capsicum annuum; Micropropagation; Phloroglucinol; Shoot bud elongation; Gibberellic acid; PLANT-REGENERATION; SWEET-PEPPER; GROWTH; PROLIFERATION; EFFICIENCY; CULTURES;

D O I：

10.1007/s40009-012-0064-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of phloroglucinol on shoot bud elongation in Capsicum annuum L. cv. Super wonder was investigated. Capsicum is recalcitrant to response under in vitro conditions and elongation of regenerated shoots has also been reported to be difficult. Shoot buds were induced from cultured cotyledons on medium supplemented with 22.2 mu M BA and 5.7 mu M IAA. The regenerated shoot buds were further elongated in the second stage on the shoot bud elongation medium containing 0.87 mu M GA(3) and 39.64 mu M PG, where the elongation occurred up to the shoot length of 4.5 cm. Healthy elongated shoots were transferred for rooting on MS medium supplemented with 2.46 mu M IBA. The well-developed plantlets were transferred to greenhouse and then into the field.

引用

页码：331 / 335

页数：5

共 26 条

[1] PLANT-REGENERATION IN TISSUE-CULTURES OF PEPPER (CAPSICUM-ANNUUM-L CV MATHANIA) [J].

AGRAWAL, S ;

CHANDRA, N ;

KOTHARI, SL .

PLANT CELL TISSUE AND ORGAN CULTURE, 1989, 16 (01) :47-55

[2] THE APPARENT INVOLVEMENT OF POLYPHENOL OXIDASE AND PHLORIDZIN IN THE PRODUCTION OF APPLE ROOTING COFACTORS [J].

BASSUK, NL ;

HUNTER, LD ;

HOWARD, BH .

JOURNAL OF HORTICULTURAL SCIENCE, 1981, 56 (04) :313-322

[3] High efficiency organogenesis in sweet pepper (Capsicum annuum L.) tissues from different seedling explants [J].

Dabauza, M ;

Peña, L .

PLANT GROWTH REGULATION, 2001, 33 (03) :221-229

[4] In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide [J].

Franck-Duchenne, M ;

Wang, YW ;

Ben Tahar, S ;

Beachy, RN .

PLANT CELL TISSUE AND ORGAN CULTURE, 1998, 53 (02) :79-84

[5]

Gaspar T, 1996, IN VITRO CELL DEV-PL, V32, P272

[6]

Gaspar T., 1991, Biotechnology in Agriculture and Forestry - High-Tech and Micropropagation I, P116, DOI [10.1007/978-3-642-76415-8_7, DOI 10.1007/978-3-642-76415-8_7]

[7]

Gomez KA, 1984, STAT PROCEDURES AGR

[8] Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. [J].

Husain, S ;

Jain, A ;

Kothari, SL .

PLANT CELL REPORTS, 1999, 19 (01) :64-68

[9]

Hyde CL, 1996, IN VITRO CELL DEV-PL, V32, P72

[10] Adventitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania coagulans (Stocks) Dunal [J].

Jain, Rohit ;

Sinha, Arunima ;

Jain, Devendra ;

Kachhwaha, Sumita ;

Kothari, S. L. .

PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 105 (01) :135-140

← 1 2 3 →