Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1 infected individuals on prolonged effective antiretroviral therapy

被引：212

作者：

Lewin, SR ^{[1
]}

Vesanen, M ^{[1
]}

Kostrikis, L ^{[1
]}

Hurley, A ^{[1
]}

Duran, M ^{[1
]}

Zhang, L ^{[1
]}

Ho, DD ^{[1
]}

Markowitz, M ^{[1
]}

机构：

[1] Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 07期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JVI.73.7.6099-6103.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

引用

收藏

页码：6099 / 6103

页数：5

相关论文

共 31 条

[1] Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo [J].

Bagnarelli, P ;

Valenza, A ;

Menzo, S ;

Sampaolesi, R ;

Varaldo, PE ;

Butini, L ;

Montroni, M ;

Perno, CF ;

Aquaro, S ;

Mathez, D ;

Leibowitch, J ;

Balotta, C ;

Clementi, M .

JOURNAL OF VIROLOGY, 1996, 70 (11) :7603-7613

[2] QUIESCENT LYMPHOCYTES-T AS AN INDUCIBLE VIRUS RESERVOIR IN HIV-1 INFECTION [J].

BUKRINSKY, MI ;

STANWICK, TL ;

DEMPSEY, MP ;

STEVENSON, M .

SCIENCE, 1991, 254 (5030) :423-427

[3] IN-VIVO FATE OF HIV-1-INFECTED T-CELLS - QUANTITATIVE-ANALYSIS OF THE TRANSITION TO STABLE LATENCY [J].

CHUN, TW ;

FINZI, D ;

MARGOLICK, J ;

CHADWICK, K ;

SCHWARTZ, D ;

SILICIANO, RF .

NATURE MEDICINE, 1995, 1 (12) :1284-1290

[4] Quantification of latent tissue reservoirs and total body viral load in HIV-1 Infection [J].

Chun, TW ;

Carruth, L ;

Finzi, D ;

Shen, XF ;

DiGiuseppe, JA ;

Taylor, H ;

Hermankova, M ;

Chadwick, K ;

Margolick, J ;

Quinn, TC ;

Kuo, YH ;

Brookmeyer, R ;

Zeiger, MA ;

BarditchCrovo, P ;

Siliciano, RF .

NATURE, 1997, 387 (6629) :183-188

[5] Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy [J].

Chun, TW ;

Stuyver, L ;

Mizell, SB ;

Ehler, LA ;

Mican, JAM ;

Baseler, M ;

Lloyd, AL ;

Nowak, MA ;

Fauci, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) :13193-13197

[6] Real-time quantitative RT-PCR after laser-assisted cell picking [J].

Fink, L ;

Seeger, W ;

Ermert, L ;

Hänze, J ;

Stahl, U ;

Grimminger, F ;

Kummer, W ;

Bohle, RM .

NATURE MEDICINE, 1998, 4 (11) :1329-1333

[7] Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy [J].

Finzi, D ;

Hermankova, M ;

Pierson, T ;

Carruth, LM ;

Buck, C ;

Chaisson, RE ;

Quinn, TC ;

Chadwick, K ;

Margolick, J ;

Brookmeyer, R ;

Gallant, J ;

Markowitz, M ;

Ho, DD ;

Richman, DD ;

Siliciano, RF .

SCIENCE, 1997, 278 (5341) :1295-1300

[8] CHANGES IN THE VIRAL MESSENGER-RNA EXPRESSION PATTERN CORRELATE WITH A RAPID RATE OF CD4(+) T-CELL NUMBER DECLINE IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE 1-INFECTED INDIVIDUALS [J].

FURTADO, MR ;

KINGSLEY, LA ;

WOLINSKY, SM .

JOURNAL OF VIROLOGY, 1995, 69 (04) :2092-2100

[9] A novel method for real time quantitative RT PCR [J].

Gibson, UEM ;

Heid, CA ;

Williams, PM .

GENOME RESEARCH, 1996, 6 (10) :995-1001

[10] Treatment with indinavir, zidovudine, and lamivudine in adults with human immunodeficiency virus infection and prior antiretroviral therapy [J].

Gulick, RM ;

Mellors, JW ;

Havlir, D ;

Eron, JJ ;

Gonzalez, C ;

McMahon, D ;

Richman, DD ;

Valentine, FT ;

Jonas, L ;

Meibohm, A ;

Emini, EA ;

Chodakewitz, JA .

NEW ENGLAND JOURNAL OF MEDICINE, 1997, 337 (11) :734-739

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