Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1 infected individuals on prolonged effective antiretroviral therapy

被引：212

作者：

Lewin, SR ^{[1
]}

Vesanen, M ^{[1
]}

Kostrikis, L ^{[1
]}

Hurley, A ^{[1
]}

Duran, M ^{[1
]}

Zhang, L ^{[1
]}

Ho, DD ^{[1
]}

Markowitz, M ^{[1
]}

机构：

[1] Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 07期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JVI.73.7.6099-6103.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

引用

页码：6099 / 6103

页数：5

共 31 条

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