High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval

被引：8

作者：

Shatalina, Margarita ^{[1
]}

Messmer, Monika ^{[2
]}

Feuillet, Catherine ^{[3
]}

Mascher, Fabio ^{[4
]}

Paux, Etienne ^{[3
]}

Choulet, Frederic ^{[3
]}

Wicker, Thomas ^{[1
]}

Keller, Beat ^{[1
]}

机构：

[1] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland

[2] Res Inst Organ Agr, CH-5070 Frick, Switzerland

[3] INRA, Genet Divers & Ecophysiol Cereals, F-63100 Clermont Ferrand, France

[4] Res Stn Agroscope Changins Wadenswil ACW, CH-1260 Nyon, Switzerland

来源：

THEORETICAL AND APPLIED GENETICS | 2014年 / 127卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

FUSARIUM HEAD BLIGHT; QUANTITATIVE TRAIT LOCUS; WINTER-WHEAT; SEPTORIA-NODORUM; PHAEOSPHAERIA-NODORUM; FIELD-RESISTANCE; INHERITANCE; GENE; LEAF; MAP;

D O I：

10.1007/s00122-013-2240-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Stagonospora nodorum glume blotch (SNG), caused by the necrotrophic fungus Stagonospora nodorum, is one of the economically important diseases of bread wheat (Triticum aestivum L.). Resistance to SNG is known to be quantitative and previous studies of a recombinant inbred line (RIL) population identified a major quantitative trait locus (QTL) for resistance to SNG on the short arm of chromosome 3B. To localize this QTL (QSng.sfr-3BS) with high resolution, we constructed a genetic map for the QTL target region using information from sequenced flow-sorted chromosomes 3B of the two parental cultivars 'Arina' and 'Forno', the physical map of chromosome 3B of cultivar 'Chinese Spring' and BAC-clone sequences. The mapping population of near-isogenic lines (NIL) was evaluated for SNG resistance in field infection tests. NILs segregated for disease resistance as well as for plant height; additionally, we observed a high environmental influence on the trait. Our analysis detected a strong negative correlation of SNG resistance and plant height. Further analysis of the target region identified two linked loci associated with SNG resistance. One of them was also associated with plant height, revealing an effect of QSng.sfr-3BS on plant height that was hidden in the RIL population. This result demonstrates an unexpectedly high genetic complexity of resistance controlled by QSng.sfr-3BS and shows the importance of the study of QTL in mendelized form in NILs.

引用

页码：573 / 586

页数：14

共 50 条

[1] Inheritance of field resistance to Stagonospora nodorum leaf and glume blotch and correlations with other morphological traits in hexaploid wheat (Triticum aestivum L.) [J].

Aguilar, V ;

Stamp, P ;

Winzeler, M ;

Winzeler, H ;

Schachermayr, G ;

Keller, B ;

Zanetti, S ;

Messmer, MM .

THEORETICAL AND APPLIED GENETICS, 2005, 111 (02) :325-336

[2] The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms. [J].

Akhunov, ED ;

Goodyear, AW ;

Geng, S ;

Qi, LL ;

Echalier, B ;

Gill, BS ;

Miftahudin ;

Gustafson, JP ;

Lazo, G ;

Chao, SM ;

Anderson, OD ;

Linkiewicz, AM ;

Dubcovsky, J ;

La Rota, M ;

Sorrells, ME ;

Zhang, DS ;

Nguyen, HT ;

Kalavacharla, V ;

Hossain, K ;

Kianian, SF ;

Peng, JH ;

Lapitan, NLV ;

Gonzalez-Hernandeiz, JL ;

Anderson, JA ;

Choi, DW ;

Close, TJ ;

Dilbirligi, M ;

Gill, KS ;

Walker-Simmons, MK ;

Steber, C ;

McGuire, PE ;

Qualset, CO ;

Dvorak, J .

GENOME RESEARCH, 2003, 13 (05) :753-763

[3] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274

[4] INHERITANCE OF SEPTORIA-GLUME BLOTCH RESISTANCE IN WHEAT [J].

BOSTWICK, DE ;

OHM, HW ;

SHANER, G .

CROP SCIENCE, 1993, 33 (03) :439-443

[5]

Broennimann A, 1975, J PLANT BREED, V75, P138

[6]

Broennimann A, 1968, MITTEIL SCHWEIZ LAND, V16, P65

[7]

Broennimann A, 1973, J PLANT BREED, V70, P230

[8] Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat [J].

Bryan, GJ ;

Stephenson, P ;

Collins, A ;

Kirby, J ;

Smith, JB ;

Gale, MD .

THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :192-198

[9] Megabase Level Sequencing Reveals Contrasted Organization and Evolution Patterns of the Wheat Gene and Transposable Element Spaces [J].

Choulet, Frederic ;

Wicker, Thomas ;

Rustenholz, Camille ;

Paux, Etienne ;

Salse, Jerome ;

Leroy, Philippe ;

Schlub, Stephane ;

Le Paslier, Marie-Christine ;

Magdelenat, Ghislaine ;

Gonthier, Catherine ;

Couloux, Arnaud ;

Budak, Hikmet ;

Breen, James ;

Pumphrey, Michael ;

Liu, Sixin ;

Kong, Xiuying ;

Jia, Jizeng ;

Gut, Marta ;

Brunel, Dominique ;

Anderson, James A. ;

Gill, Bikram S. ;

Appels, Rudi ;

Keller, Beat ;

Feuillet, Catherine .

PLANT CELL, 2010, 22 (06) :1686-1701

[10]

Crawley M. J., 2007, R BOOK, DOI DOI 10.1002/9780470515075

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