CD8-alpha T-cell infiltration in human papillomavirus-related oropharyngeal carcinoma correlates with improved patient prognosis

被引:81
作者
Jung, Alain C. [1 ]
Guihard, Sebastien [1 ,2 ]
Krugell, Sylvie [1 ]
Ledrappier, Sonia [1 ]
Brochot, Alexandra [3 ]
Dalstein, Veronique [3 ]
Job, Sylvie [4 ]
de Reynies, Aurelien [4 ]
Noel, Georges [1 ,2 ]
Wasylyk, Bohdan [5 ]
Clavel, Christine [3 ]
Abecassis, Joseph [1 ]
机构
[1] Ctr Reg Lutte Canc Paul Strauss, EA3430, Lab Biol Tumorale, F-67065 Strasbourg, France
[2] Ctr Reg Lutte Canc Paul Strauss, Dept Radiotherapie, F-67065 Strasbourg, France
[3] CHU Reims, Lab Pol Bouin, INSERM, UMRS 903, Reims, France
[4] Ligue Natl Canc, Programme Cartes Identite Tumeurs CIT, Paris, France
[5] UDS, CNRS, INSERM, IGBMC,U964,UMR7104, Illkirch Graffenstaden, France
关键词
human papillomavirus; oropharyngeal cancer; lymphocyte infiltration; CD8; T-cell; prognosis; HEAD; EXPRESSION; SURVIVAL; CANCER; E7; INDUCTION; INFECTION; IMMUNITY; GENES; E6;
D O I
10.1002/ijc.27776
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Patients with human papillomavirus (HPV)-related oropharyngeal tumors display improved prognosis. The biological basis of this tumor phenotype is poorly understood. We investigated whether increased lymphocyte infiltrate in HPV-positive oropharyngeal squamous cell carcinomas could account for better prognosis. We previously identified, in an Affymetrix GeneChip analysis of 83 HPV-unrelated and 11 HPV-related squamous cell carcinoma of the oropharynx, several candidate genes, including CD8a and CD3?. Their expression was validated in this study by qRTPCR on an independent clinical series of 144 oropharyngeal tumors. Immunohistochemical staining of tumor specimens was performed to evaluate infiltration of tumor stroma by CD8+ and CD4+ lymphocytes. The prognostic value of CD8a and CD3? expression levels was measured by KaplanMeier and Cox regression model analyses. Immune response-related signaling pathways were found to be deregulated in HPV-positive oropharyngeal tumors. Expression of CD8a, CD3?, granzyme K, CD28 and integrin aL RNAs was upregulated in HPV-positive lesions when compared with HPV-unrelated tumors (p < 0.05). Stroma of HPV-positive tumors was frequently and strongly infiltrated by CD8a- and CD3?-positive T cells. CD8a RNA expression correlated with both improved global (KaplanMeier; p = 0.005; Cox regression: p = 0.003) and disease-free (Cox regression: p = 0.04) survival. CD3? RNA expression correlated with improved overall survival (Cox regression: p = 0.024). These results suggest that an increased cytotoxic T-cell-based antitumor immune response is involved in improved prognosis of patients with HPV-positive tumors.
引用
收藏
页码:E26 / E36
页数:11
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