GC-MS based targeted metabolomics approach for studying the variations of phenolic metabolites in artificially ripened banana fruits

被引:20
作者
Tallapally, Maheshwari [1 ,2 ]
Sadiq, Asif Shaikh [2 ]
Mehtab, Vazida [1 ,3 ]
Chilakala, Shireesha [1 ,2 ]
Vemula, Madhu [1 ,2 ]
Chenna, Sumana [1 ,3 ]
Upadhyayula, Vijayasarathi [1 ,2 ]
机构
[1] Acad Sci & Innovat Res, Ghaziabad 201002, Uttar Pradesh, India
[2] CSIR Indian Inst Chem Technol, Ctr Mass Spectrometry, Analyt Div, Hyderabad 500007, India
[3] CSIR Indian Inst Chem Technol, Proc Engn & Technol Transfer, Hyderabad 500007, India
关键词
Banana; Artificial ripening; Phenolic compounds; Gas chromatography-mass spectrometry; Ethephon; Calcium carbide; ETHYLENE; PEEL; MUSA;
D O I
10.1016/j.lwt.2020.109622
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The variations of volatile phenolic metabolites in the artificially ripened banana fruits (Musa Sapient um) were studied. The fruits were artificially ripened using calcium carbide, ethephon (Ethrel) solution, and ethylene gas. The phenolics were extracted from the fruit pulp and analysed using gas chromatography-mass spectrometry. The obtained phenolic compounds were quantified with reference to the concentration of 1,3-dihydroxybenzene. The results showed significant upregulation of sinapic acid, butylated hydroxytoluene, a-hydroxycinnamic acid, isoferulic acid, and downregulation of 2,4,6-trimethoxytoluene and elemicin in the fruits ripened with calcium carbide. Ripening with ethylene gas showed upregulation of vanillic acid and the ripening by dipping the fruits in ethephon solution showed up-regulation of 2,4,6-trimethoxytoluene, p-coumaric acid and downregulation of isoferulic acid. The data was subjected to analysis of variance (ANOVA) and principal component analysis (PCA). The ANOVA analysis statistically certified the significance of variation among different ripening methods and the quantified phenolic metabolites. The results obtained from the PCA studies indicate that the artificially ripened banana fruits with ethylene and ethephon exhibited distinct patterns as compared to that of the naturally ripened ones.
引用
收藏
页数:8
相关论文
共 33 条
[1]  
Abdelaziz FH, 2000, INFLUENCE VARYING MA
[2]  
ABOUAZIZ AB, 1970, CURR SCI INDIA, V39, P552
[3]  
Aboul-Enein A. M., 2016, Journal of Chemical and Pharmaceutical Research, V8, P46
[4]   Effect of ethephon on the liver in albino rats: A histomorphometric study [J].
Bhadoria, Pooja ;
Nagar, Mahindra ;
Bahrioke, Veena ;
Bhadoria, Ajeet Singh .
BIOMEDICAL JOURNAL, 2015, 38 (05) :421-427
[5]   Physicochemical and nutritional characteristics of banana flour during ripening [J].
Campuzano, Ana ;
Rosell, Cristina M. ;
Cornejo, Fabiola .
FOOD CHEMISTRY, 2018, 256 :11-17
[6]   NMR and LC-MS assessment of compound variability of common bean (Phaseolus vulgaris) stored under controlled atmosphere [J].
Coelho, Silvia Renata M. ;
Alves Filho, Elenilson G. ;
Silva, Lorena Mara A. ;
Bischoff, Tabata Z. ;
Ribeiro, Paulo R., V ;
Zocolo, Guilherme Juliao ;
Canuto, Kirley M. ;
Bassinello, Priscila Z. ;
de Brito, Edy S. .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2020, 117 (117)
[7]  
Das B. C., 2011, J TEACHING RES CHEM, V18, P22
[8]  
Dighe S. B., 2010, PHARMACOLOGYONLINE, V1, P491
[9]  
Fatemeh S. R., 2012, International Food Research Journal, V19, P1041
[10]  
Hossain MF, 2015, INT FOOD RES J, V22, P1755