PREPARATIVE SEPARATION AND PURIFICATION OF BUFADIENOLIDES FROM ChanSu BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY COMBINED WITH PREPARATIVE HPLC

被引：0

作者：

Li, Jialian ^{[1
]}

Zhang, Yongqing ^{[1
]}

Lin, Yunliang ^{[2
]}

Wang, Xiao ^{[2
]}

Fang, Lei ^{[2
]}

Geng, Yanling ^{[2
]}

Zhang, Qinde ^{[3
]}

机构：

[1] Shandong Univ Tradit Chinese Med, Coll Pharm, Jinan 250355, Shandong, Peoples R China

[2] Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China

[3] Shandong Coll Tradit Chinese Med, Laiyang 265200, Shandong, Peoples R China

来源：

QUIMICA NOVA | 2013年 / 36卷 / 05期

关键词：

high-speed counter-current chromatography; bufadienolides; preparative HPLC; CINOBUFAGIN; MEDICINE; BUFALIN;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Eight bufadienolides were successfully isolated and purified from ChanSu by high-speed counter-current chromatography (HSCCC) combined with preparative HPLC (prep-HPLC). First, a stepwise elution mode of HSCCC with the solvent system composed of petroleum ether-ethyl acetate-methanol-water (4: 6: 4: 6, 4: 6: 5: 5, v/v) was employed and four bufadienolides, two partially purified fractions were obtained from 200 mg of crude extract. The partially purified fractions III and VI were then further separated by prep-HPLC, respectively, and another four bufadienolides were recovered. Their structures were confirmed by ESI-MS and H-1-NMR spectra.

引用

页码：686 / 690

页数：5

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