Silver-nano biohybride material: Synthesis, characterization and application in water purification

被引:105
作者
Das, Sujoy K. [1 ]
Khan, Md. Motiar R. [2 ]
Guha, Arun K. [2 ]
Das, Akhil R. [3 ]
Mandal, Asit Baran [1 ]
机构
[1] Cent Leather Res Inst, CSIR, Environm Technol Lab, Madras 600020, Tamil Nadu, India
[2] Indian Assoc Cultivat Sci, Dept Biol Chem, Kolkata 700032, India
[3] Indian Assoc Cultivat Sci, Polymer Sci Unit, Kolkata 700032, India
关键词
Silver nanoparticles; Rhizopus oryzae; Pesticide adsorption; Antibacterial activity; ADSORPTION BEHAVIOR; NANOPARTICLES; GOLD; EXTRACTS;
D O I
10.1016/j.biortech.2012.08.071
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A green chemical synthesis of silver nanoparticles (AgNPs) through in situ reduction of silver nitrate (AgNO3) by a fungal strain of Rhizopus oryzae is described along with the promising eco-friendly role of the synthesized nano-silver bioconjugate (NSBC) material in water purification process. The NSBC has been characterized using UV-vis spectroscopy, high resolution transmission electron (HRTEM) microscopy, and Fourier transform infrared (FTIR) spectroscopy. The NSBC exhibits strong antibacterial activity against Escherichia coli and Bacillus subtilis and high adsorption capacity towards different organophosphorous pesticides. Fluorescence and electron microscopic images reveal NSBC binds on the bacterial cell wall, which cause irreversible membrane damage eventually leading to cell death. Proteomic analysis further demonstrates down regulation of protein expression, inhibition of cytosolic and membrane proteins and leakage of cellular content following binding of NSBC with bacterial cell wall. NSBC has been exploited to obtain potable water free from pathogens and pesticides in one step process. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:495 / 499
页数:5
相关论文
共 22 条
[1]   Toward greener nanosynthesis [J].
Dahl, Jennifer A. ;
Maddux, Bettye L. S. ;
Hutchison, James E. .
CHEMICAL REVIEWS, 2007, 107 (06) :2228-2269
[2]   Adsorption behavior of rhodamine B on Rhizopus oryzae biomass [J].
Das, Sujoy K. ;
Bhowal, Jayati ;
Das, Akhil R. ;
Guha, Arun K. .
LANGMUIR, 2006, 22 (17) :7265-7272
[3]   Synthesis, characterization and catalytic activity of gold nanoparticles biosynthesized with Rhizopus oryzae protein extract [J].
Das, Sujoy K. ;
Dickinson, Calum ;
Lafir, Fathima ;
Brougham, Dermot F. ;
Marsili, Enrico .
GREEN CHEMISTRY, 2012, 14 (05) :1322-1334
[4]   A green chemical approach for the synthesis of gold nanoparticles: characterization and mechanistic aspect [J].
Das, Sujoy K. ;
Marsili, Enrico .
REVIEWS IN ENVIRONMENTAL SCIENCE AND BIO-TECHNOLOGY, 2010, 9 (03) :199-204
[5]   Adsorption Behavior of Mercury on Functionalized Aspergillus versicolor Mycelia: Atomic Force Microscopic Study [J].
Das, Sujoy K. ;
Das, Akhil R. ;
Guha, Arun K. .
LANGMUIR, 2009, 25 (01) :360-366
[6]  
Feng QL, 2000, J BIOMED MATER RES, V52, P662, DOI 10.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO
[7]  
2-3
[8]   Adsorption behavior of lindane on Rhizopus oryzae biomass: Physico-chemical studies [J].
Ghosh, Subrata ;
Das, Sujoy K. ;
Guha, Arun K. ;
Sanyal, Asis K. .
JOURNAL OF HAZARDOUS MATERIALS, 2009, 172 (01) :485-490
[9]   Nanotechnology and the challenge of clean water [J].
Hillie, Thembela ;
Hlophe, Mbhuti .
NATURE NANOTECHNOLOGY, 2007, 2 (11) :663-664
[10]   Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli [J].
Li, Wen-Ru ;
Xie, Xiao-Bao ;
Shi, Qing-Shan ;
Zeng, Hai-Yan ;
Ou-Yang, You-Sheng ;
Chen, Yi-Ben .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) :1115-1122