Development of real-time immuno-PCR for the quantitative detection of mycobacterial PstS1 in tuberculosis patients

被引：15

作者：

Sharma, Suman ^{[1
]}

Raj, Ankush ^{[1
]}

Singh, Netrapal ^{[1
]}

Dahiya, Bhawna ^{[1
]}

Sheoran, Abhishek ^{[2
]}

Gupta, Krishna B. ^{[3
]}

Mehta, Promod K. ^{[1
]}

机构：

[1] Maharshi Dayanand Univ, Ctr Biotechnol, Rohtak 124001, Haryana, India

[2] Amity Univ, Amity Inst Appl Sci, Dept Stat, Noida 201303, India

[3] Univ Hlth Sci, Dept TB & Resp Med, Rohtak 124001, Haryana, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 132卷

关键词：

Mycobacterium tuberculosis; Tuberculosis; Real-time immuno-PCR; PstS1; Diagnosis; EXTRAPULMONARY TUBERCULOSIS; AMPLIFIED IMMUNOASSAY; ANTIGEN; 85B; DIAGNOSIS; SERODIAGNOSIS; ASSAY; METAANALYSIS; ESAT-6; TESTS;

D O I：

10.1016/j.mimet.2016.12.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel indirect real-time immuno-polymerase chain reaction (RT-I-PCR) assay, an evolution of I-PCR, was developed for the quantitative detection of Mycobacterium tuberculosis PstS1 (Rv0934) with a wide dynamic range of 10 ng/mL to 1 pg/mL in body fluids of tuberculosis (TB) patients, which may monitor the dynamics of disease. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：134 / 138

页数：5

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