Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase

被引：45

作者：

Kumar, Vinod ^{[1
]}

Sankaranarayanan, Mugesh ^{[1
]}

Durgapal, Meetu ^{[1
]}

Zhou, Shengfang ^{[1
]}

Ko, Yeounjoo ^{[1
]}

Ashok, Somasundar ^{[1
]}

Sarkar, Ritam ^{[1
]}

Park, Sunghoon ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Chem & Biomol Engn, Pusan 609735, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 135卷

关键词：

K. pneumoniae J2B KGSADH; K. pneumoniae J2B Delta ldhA KGSADH; 3-Hydroxypropionic acid; 1,3-Propanediol; Lactate dehydrogenase; ESCHERICHIA-COLI; STRAIN; FERMENTATION; TOLERANCE; GENOME;

D O I：

10.1016/j.biortech.2012.11.018

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In the present study, the lactate dehydrogenase-deficient (ldhA(-)) recombinant Klebsiella pneumoniae overexpressing an ALDH (KGSADH) was developed and the co-production of 3-HP and PDO from glycerol by this recombinant under resting cell conditions was examined. The new recombinant did not produce any appreciable lactate, which seriously inhibits the production of 3-HP and PDO. The final titers of 3-HP and PDO by the ldhA(-) recombinant strain at 60 h were 252.2 mM and 308.7 mM, respectively, which were improved by approximately 30% and 50%, respectively, compared to those by the counterpart recombinant strain, which was the wild type for ldhA. In addition, after deleting ldhA, the cumulative yield on glycerol and specific production rate of these two metabolites (3-HP and PDO) were enhanced by 41.4% and 52%, respectively. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：555 / 563

页数：9

共 25 条

[1] Inhibition of Succinic Acid Production in Metabolically Engineered Escherichia Coli by Neutralizing Agent, Organic Acids, and Osmolarity [J].