Head and flagella subcompartmental proteomic analysis of human spermatozoa

被引：110

作者：

Baker, Mark A. ^{[1
]}

Naumovski, Nenad ^{[1
]}

Hetherington, Louise ^{[1
]}

Weinberg, Anita ^{[1
]}

Velkov, Tony ^{[2
]}

Aitken, R. John ^{[1
]}

机构：

[1] Univ Newcastle, Prior Res Ctr Reprod Sci, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia

[2] Monash Univ, Monash Inst Pharmaceut Sci, Parkville, Vic, Australia

来源：

PROTEOMICS | 2013年 / 13卷 / 01期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Antioxidant; Cell biology; ESI-MS; MS; Spermatogenesis; Superoxide; Tandem mass spectra; PROTEIN-TYROSINE PHOSPHORYLATION; SPERM CELL BIOLOGY; IPG STRIP PREFRACTIONATION; OUTER ACROSOMAL MEMBRANE; LC-MS/MS IDENTIFICATION; EPIDIDYMAL MATURATION; MOUSE SPERMATOZOA; MAMMALIAN SPERM; MALE-FERTILITY; SIGNALING PATHWAYS;

D O I：

10.1002/pmic.201200350

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Subcellular proteomics not only deepens our knowledge of what proteins are present within cells, but also opens our understanding as to where those proteins reside. Given the highly differentiated, cross-linked state of spermatozoa, such studies have proven difficult to perform. In this study we have fractionated spermatozoa into two components, consisting of either the head or flagellar region. Following SDS-PAGE, 1 mm slices were digested and used for LC-MS/MS analysis. In total, 1429 proteins were identified with 721 proteins being exclusively found in the tail and 521 exclusively in the head. Not only is this the largest reported proteomic analysis of human spermatozoa, but also it has provided novel insights into the compartmentalization of proteins, particularly receptors, never previously reported to be present in this cell type.

引用

页码：61 / 74

页数：14

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