Quantitative assessment of the relative contributions of steric repulsion and chemical interactions to macromolecular crowding

被引：115

作者：

Minton, Allen P. ^{[1
]}

机构：

[1] NIDDKD, Sect Phys Biochem, Lab Biochem & Genet, NIH,US Dept HHS, Bethesda, MD 20892 USA

来源：

BIOPOLYMERS | 2013年 / 99卷 / 04期

关键词：

excluded volume; steric repulsion; weak binding; equivalent hard particle model; BOVINE SERUM-ALBUMIN; PROTEIN-PROTEIN; MODEL; VOLUME; ASSOCIATION; POLYMER;

D O I：

10.1002/bip.22163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The term macromolecular crowding denotes the combined effects of high volume fractions of nominally unrelated macromolecules upon the equilibrium and transport properties of all macrosolutes, dilute as well as concentrated, in the crowded medium. We present a formal partitioning of the total crowding effect into contributions from steric exclusion (excluded volume) and weak, nonspecific attractive interactions between a concentrated crowding agent and reactant and product species present at trace concentration. A numerical example of the combined effect of both steric and chemical interactions between crowder and tracer upon the reversible dimerization of tracer is presented, based upon reasonable estimates of the magnitude of both repulsive and attractive interactions between tracer and crowder species. (c) 2012Wiley Periodicals, Inc. Biopolymers 99: 239244, 2013.

引用

页码：239 / 244

页数：6

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