Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors

Taking advantage of the excellent catalytic redox activity andthe electrochemical property of DNAzyme and carbon nanotubes, a novel nano-complex of single-walled carbon nanotubes (SWNTs)/DNAzyme was prepared, and the obtained nano-complex was finally immobilized on the surface of glass carbon electrode(GCE) with the assistance of chitosan to fabricate electrochemical biosensing interface. the catalytic property of the SWNTs/DNAzyme complex was explored, and a glucose oxidase biosensor was then prepared based on the fabricated electrochemical biosensing platform. the SWNTs/DNAzyme complex-based biosensing platform can direct response to hydrogen peroxide at low potential of -0.24 V with high sensitivity (the detection limit is 1x10(-6) mol/L) and wide linear range (5x10(-6)-1x10(-2) mol/L), and the prepared glucose oxidase biosensor was used to fast and sensitive detection of glucose.