BIOSYNTHESIS OF SILVER NANOPARTICLES USING LATEX OF SYANDENIUM GRANTII HOOK F AND ITS ASSESSMENT OF ANTIBACTERIAL ACTIVITIES

We report hereby a green synthesis method employing latex of Syandenium grantii Hook f to biosynthesize silver nanoparticles in presence of sunlight irradiation. Silver nanoparticles with antibacterial potencies are of great interest in the biomedical field. Biogenic silver nanoparticles were biosynthesized by using latex of S. grantii and were characterized in Fourier Transform Infra Red Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). In antibacterial studies, Kirby-Bauer disk diffusion susceptibility test was used to measure efficacy of biogenic silver nanoparticles against clinical isolates of Gram positive and Gram negative bacteria strains available from hospital like Staphylococcus aureus, Streptococcus pyogens, Escherichia coli and Pseudomonas aeruginosa in terms of zone inhibition. Biogenic silver nanoparticles have possessing different desirable physicochemical properties owing in polydispersity, presence of biomolecules capping on the surface of silver nanoparticles and its particle size was found to be nanoscale below 50nm. Various peaks in FTIR denote the different functional groups present in the latex of Syandenium grantii acts as capping agent. From SEM result, it has shown the agglomerates of biogenic silver nanoparticles. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 14-35 nm. Antibacterial activity was measured. Efficacy of biogenic silver nanoparticles against bacterial strains was found in ascending order of P. aeruginosa > S. aureus > E. coli > S. pyogens. A green approach for development of silver nanoparticles using latex of S. grantii appears an ease, economic, ecofriendly and expressive biosynthesis with aid of sunlight irradiation.