Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings

被引：22
作者：
Bozari, Sedat
Agar, Guleray [1 ]
Aksakal, Ozkan [1 ]
Erturk, Filiz A. [1 ]
Yanmis, Derya [1 ]
机构：
[1] Mus Alparslan Univ, Dept Biol, TR-49100 Mus, Turkey
来源：
TOXICOLOGY AND INDUSTRIAL HEALTH | 2013年 / 29卷 / 04期
关键词：
Genotoxic; catmint; GC-MS; RAPD; SDS-PAGE; allelopathy; ANTIBACTERIAL ACTIVITY; METHANOL EXTRACTS; SEED-GERMINATION; MEYERI BENTH; L; ANTIOXIDANT; NEPETALACTONE; BOISS; RAPD;
D O I：
10.1177/0748233711433939
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
We aimed to determine the genotoxic potential of essential oil (EO) obtained from Nepeta nuda. The chemical content of EO was measured via gas chromatography/mass spectrometry. The most abundant contents were 4a alpha,7 beta,7a alpha-nepetalactone (18.10%), germacrene (15.68%) and elemol (14.38%). For genotoxic effects of EO, Zea mays' seeds were exposed to four different concentrations of this oil. Inhibition of root and stem growth were observed with an increase in EO concentrations. Randomly amplified polymorphic DNA (RAPD) method was used to determine the genotoxic effects of EO. Some changes occurred in RAPD profiles of germinated EO-treated seeds. Even though total soluble protein quantity vary, the data observed from the protein profiles of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that there was a little differentiation between band profiles of treated samples and control group. We concluded that the basis of interactions between plants, like allelopathy, may be related with genotoxic effects of EO.
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收藏
页码：339 / 348
页数：10
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