Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast

被引：6

作者：

Tanae, Katsuhiro ^{[1
]}

Horiuchi, Tomitaka ^{[1
]}

Yamakawa, Takuya ^{[1
]}

Matsuo, Yuzy ^{[1
]}

Kawamukai, Makoto ^{[1
]}

机构：

[1] Shimane Univ, Dept Appl Biosci & Biotechnol, Fac Life & Environm Sci, Matsue, Shimane 6908504, Japan

来源：

FEBS LETTERS | 2012年 / 586卷 / 23期

关键词：

Fission yeast; Histone chaperone; Centromere; Asf1; CENP-A; CENP-A; CENTROMERIC CHROMATIN; PROTEIN; SCM3; KINETOCHORE; DEPOSITION; REPAIR;

D O I：

10.1016/j.febslet.2012.10.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Delta sim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Delta sim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Delta sim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：4190 / 4196

页数：7

共 22 条

[1] Epigenetic regulation of centromeric chromatin: old dogs, new tricks? [J].