Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment

被引：21
作者：
Coffinier, Yannick [1 ]
Nguyen, Nhung [1 ,2 ]
Drobecq, Herve [3 ]
Melnyk, Oleg [3 ]
Thomy, Vincent [2 ]
Boukherroub, Rabah [1 ]
机构：
[1] Univ Lille Nord France, IRI, CNRS, USR 3078, F-59658 Villeneuve Dascq, France
[2] Univ Lille Nord France, IEMN, UMR 8520, CNRS, F-59652 Villeneuve Dascq, France
[3] Univ Lille Nord France, IBL, CNRS, UMR 8161, F-59021 Lille, France
来源：
ANALYST | 2012年 / 137卷 / 23期
关键词：
DESORPTION-IONIZATION; POROUS SILICON; MATRIX; MONOLAYERS; NANOWIRE; NANOPARTICLES; ATTACHMENT; CHEMISTRY; PROTEINS; TARGET;
D O I：
10.1039/c2an35803d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this paper, we report on the functionalization of silicon nanostructured (NanoSi) surface with an organic layer of nitrilotriacetic acid (NTA) and its subsequent use as an affinity surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) interface for histidine-tagged peptide enrichment and mass spectrometry analysis. The NTA terminal groups are immobilized onto the NanoSi surface via very stable Si-C covalent bonds. The NTA-modified NanoSi (NTA-NanoSi) interface was characterized by contact angle measurements, Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The NTA-NanoSi interface has shown a good selectivity toward His-tagged peptide and permits its enrichment from an artificial mixture of both tagged and untagged peptides and its subsequent mass spectrometry detection with good signal/noise ratio.
引用
收藏
页码：5527 / 5532
页数：6
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