Genetic diversity of Fusarium graminearum sensu lato isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina

被引：10
作者：
Consolo, Veronica F. [1 ]
Ortega, Leonel M. [2 ]
Salerno, Graciela [1 ]
Astoreca, Andrea L. [2 ]
Alconada, Teresa M. [2 ]
机构：
[1] Consejo Nacl Invest Cient & Tecn, INBIOTEC Inst Invest Biodiversidad & Biotecnol, Mar Del Plata, Buenos Aires, Argentina
[2] Natl Univ La Plata, Fac Ciencias Exactas, Ctr Invest & Desarrollo Fermentac Ind CINDEFI, CCT La Plata,CONICET, La Plata, Buenos Aires, Argentina
来源：
REVISTA ARGENTINA DE MICROBIOLOGIA | 2015年 / 47卷 / 03期
关键词：
Intergenic Spacer-Restriction Fragment Length Polymorphism; Inter-simple sequence repeat; Fusarium Head Blight; Fusarium graminearum; Wheat; REGION; CULMORUM; CEREALS;
D O I：
10.1016/j.ram.2015.05.004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers showed genetic variability among the analyzed isolates; however, IGS-RFLP was more efficient than ISSR, showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages of polymorphic information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability of F. graminearum isolates from wheat in Argentina using IGS-RFLP and ISSR markers. (C) 2015 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U.
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页码：245 / 250
页数：6
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