Invaders: Recognition of Double-Stranded DNA by Using Duplexes Modified with Interstrand Zippers of 2-O-(Pyren-1-yl)methyl-ribonucleotides

被引：16

作者：

Didion, Bradley A. ^{[1
]}

Karmakar, Saswata ^{[2
]}

Guenther, Dale C. ^{[2
]}

Sau, Sujay P. ^{[2
]}

Verstegen, John P. ^{[1
]}

Hrdlicka, Patrick J. ^{[2
]}

机构：

[1] Minitube Amer Inc, Verona, WI 53593 USA

[2] Univ Idaho, Dept Chem, Moscow, ID 83844 USA

来源：

CHEMBIOCHEM | 2013年 / 14卷 / 13期

基金：

美国国家卫生研究院;

关键词：

DNA recognition; FISH; fluorescent probes; nucleic acid hybridization; oligonucleotides; PEPTIDE NUCLEIC-ACIDS; TRIPLEX-FORMING OLIGONUCLEOTIDES; HELICAL B-DNA; CHROMOSOMAL DNA; FUNCTIONALIZED 2'-AMINO-ALPHA-L-LNA; MIXED-SEQUENCE; TARGETING DNA; Y-CHROMOSOME; INVASION; PYRENE;

D O I：

10.1002/cbic.201300414

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The invasion has begun: Invaders are shown to recognize DNA hairpins in cell-free assays and chromosomal DNA during non-denaturing fluorescence in situ hybridization (nd-FISH) experiments. As Invaders are devoid of inherent sequence limitations, many previously inaccessible DNA targets could become accessible to exogenous control with important ramifications for karyotyping, in vivo imaging, and gene regulation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1534 / 1538

页数：5

共 45 条

[1] Artificial DNA cutters for DNA manipulation and genome engineering [J].