Bacillus oryzisoli sp nov., isolated from rice rhizosphere

The taxonomy of strain 1DS3-10(T), a Gram-staining-positive, endospore-forming bacterium isolated from rice rhizosphere, was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel strain was grouped with established members of the genus Bacillus and appeared to be closely related to the type strains Bacillus benzoevorans DSM 5391(T) (97.9 %), Bacillus circulans DSM 11(T) (97.7 %), Bacillus novalis JCM 21709(T) (97.3 %), Bacillus soli JCM 21710(T) (97.3 %), Bacillus oceanisediminis CGMCC 1.10115(T) (97.3 %) and Bacillus nealsonii FO-92(T) (97.1 %). The fatty acid profile of strain 1DS3-10(T), which showed a predominance of iso-C-15 : 0 and anteiso-C-15 : 0, supported the allocation of the strain to the genus Bacillus. The predominant menaquinone was MK-7 (100 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminolipids. Cell-wall peptidoglycan contained mesodiaminopimelic acid. DNA-DNA hybridization values between strain 1DS3-10(T) and the type strains of closely related species were 25-33 %, which supported that 1DS3-10(T) represented a novel species in the genus Bacillus. The results of some physiological and biochemical tests also allowed the phenotypic differentiation of strain 1DS3-10(T) from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 1DS3-10(T) represents a novel species of the genus Bacillus, for which the name Bacillus oryzisoli sp. nov. is proposed. The type strain of the novel species is 1DS3-10(T) (=ACCC 19781(T) =DSM 29761(T)).