Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus

被引：38

作者：

Yang, Guoliang ^{[2
]}

Li, Bing ^{[1
]}

Liu, Lanzheng ^{[2
]}

Huang, Weichu ^{[1
]}

Zhang, Wen ^{[2
]}

Liu, Yuandong ^{[1
]}

机构：

[1] Jinan Junqu Ctr Dis Control & Prevent, Jinan 250014, Shandong, Peoples R China

[2] Jinan Ctr Dis Control & Prevent, Jinan 250021, Shandong, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 09期

关键词：

Clinical diagnosis; Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV); Reverse transcription loop-mediated isothermal amplification (RT-LAMP); Family Bunyaviridae; VISUAL DETECTION; VIRUS; FEVER; LAMP;

D O I：

10.1007/s00705-012-1348-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The etiological agent of severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus that was first identified in China in 2009. We have developed and validated a one-step, single-tube, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SFTS bunyavirus (SFTSV). This assay demonstrated high specificity and sensitivity, with a detection limit of 10(1) TCID50 ml(-1). When combined with the fluorescent detection reagent (FDR) method, results could be determined by observing a color change within 30 min. As an accurate, rapid, simple and low-cost diagnostic method, this RT-LAMP assay will be helpful for detecting and preventing further SFTSV infection in China.

引用

页码：1779 / 1783

页数：5

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