The NLRP3 rs10754558 polymorphism is a risk factor for preeclampsia in a Chinese Han population

Objective: Previous studies have indicated that the nucleotide-binding domain, leucine-rich repeat containing protein 3 (NLRP3) inflammasome is activated by monosodium urate in the trophoblast of preeclampsia (PE) patients, leading to augmented placental IL-1 levels. Thus, the purpose of our study was to investigate the association between NLRP3 polymorphisms, rs10754558 and rs2027432, and PE in Chinese Han population.Methods: The NLRP3 polymorphisms, rs10754558 and rs2027432, were genotyped by real-time PCR in 1024 PE patients and 1194 control subjects. A (2) test was used to compare the genetic distribution between the two groups, and an analysis of variance was used to conduct the genotype-phenotype analysis.Results: We demonstrated a significant difference in genotypic frequency of the rs10754558 ((2)=9.97, p=.007) in NLRP3 between PE patients and controls. Additionally, there was a significant difference between cases and controls in the dominant model of G allele ((2)=7.70, p=.006, odds ratio =0.77, 95%confidence interval 0.64-0.93). What's more, the genotypes distributions of rs10754558 were found to be associated with both the severe and late onset PE. ((2)=8.53 p=.01, (2)=9.24, p=.01.) However, no significant statistic differences were found in the genotypic distributions and allelic frequencies for rs2027432 between two groups (for genotypic distribution, (2)=0.17, p=.92; for allelic frequency, (2)=2.26, p=.13, odds ratio =0.90, 95% confidence interval 0.79-1.03).Conclusions: Our results reveal that NLRP3 may be involved in the development of PE in a Chinese Han population. However, further validation of the associations of other NLRP3 SNPs with PE in other populations is required.