Contiguous RNA editing sites in the mitochondrial nad1 transcript of Arabidopsis thaliana are recognized by different proteins

被引：15

作者：

Arenas-M, Anita ^{[1
]}

Takenaka, Mizuki ^{[2
]}

Moreno, Sebastian ^{[1
]}

Gomez, Isabel ^{[1
]}

Jordana, Xavier ^{[1
]}

机构：

[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Genet Mol & Microbiol, Santiago, Chile

[2] Univ Ulm, D-89069 Ulm, Germany

来源：

FEBS LETTERS | 2013年 / 587卷 / 07期

关键词：

RNA editing factor; Plant mitochondria; Pentatricopeptide repeat protein; PPR protein; nad1; MEF25; PENTATRICOPEPTIDE REPEAT PROTEIN; COMPLEX-II; DYW DOMAIN; GENE; REVEALS; GENOME;

D O I：

10.1016/j.febslet.2013.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentatricopeptide repeat (PPR) proteins have been identified as site-specific factors for RNA editing in plant organelles. These proteins recognize cis-elements near the editing site. It is unclear how contiguous sites are addressed, and whether one or two factors are required. We here show the PPR MEF25 to be essential for RNA editing at the nad1-308 site in Arabidopsis mitochondria. Another editing site just one nucleotide upstream, nad1-307, is edited normally in mef25 mutant lines. This finding shows that two independent factors recognizing similar cis-elements are involved at these contiguous sites without competing with each other in vivo. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：887 / 891

页数：5

共 28 条

[1] A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins [J].

Barkan, Alice ;

Rojas, Margarita ;

Fujii, Sota ;

Yap, Aaron ;

Chong, Yee Seng ;

Bond, Charles S. ;

Small, Ian .

PLOS GENETICS, 2012, 8 (08)

[2] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[3] A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during arabidopsis seed development and germination [J].

Elorza, A ;

Roschzttardtz, H ;

Gómez, I ;

Mouras, A ;

Holuigue, L ;

Araya, A ;

Jordana, X .

PLANT AND CELL PHYSIOLOGY, 2006, 47 (01) :14-21

[4] cis recognition elements in plant mitochondrion RNA editing [J].

Farré, JC ;

Leon, G ;

Jordana, X ;

Araya, A .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (20) :6731-6737

[5] The evolution of RNA editing and pentatricopeptide repeat genes [J].

Fujii, Sota ;

Small, Ian .

NEW PHYTOLOGIST, 2011, 191 (01) :37-47

[6] RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs [J].

Giegé, P ;

Brennicke, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15324-15329

[7] A unique transcriptome: 1782 positions of RNA editing alter 1406 codon identities in mitochondrial mRNAs of the lycophyte Isoetes engelmannii [J].

Grewe, Felix ;

Herres, Stefan ;

Viehoever, Prisca ;

Polsakiewicz, Monika ;

Weisshaar, Bernd ;

Knoop, Volker .

NUCLEIC ACIDS RESEARCH, 2011, 39 (07) :2890-2902

[8] The Pentatricopeptide Repeat Protein OTP87 Is Essential for RNA Editing of nad7 and atp1 Transcripts in Arabidopsis Mitochondria [J].

Hammani, Kamel ;

des Francs-Small, Catherine Colas ;

Takenaka, Mizuki ;

Tanz, Sandra K. ;

Okuda, Kenji ;

Shikanai, Toshiharu ;

Brennicke, Axel ;

Small, Ian .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (24) :21361-21371

[9] A Study of New Arabidopsis Chloroplast RNA Editing Mutants Reveals General Features of Editing Factors and Their Target Sites [J].

Hammani, Kamel ;

Okuda, Kenji ;

Tanz, Sandra K. ;

Chateigner-Boutin, Anne-Laure ;

Shikanai, Toshiharu ;

Small, Ian .

PLANT CELL, 2009, 21 (11) :3686-3699

[10] Recombinational cloning with plant gateway vectors [J].

Karimi, Mansour ;

Depicker, Ann ;

Hilson, Pierre .

PLANT PHYSIOLOGY, 2007, 145 (04) :1144-1154

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