Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening

被引：33

作者：

Zhou, Tao ^{[2
,3
,4
,5
]}

Zhang, Hang ^{[2
,3
,6
]}

Lai, Tongfei ^{[1
]}

Qin, Cheng ^{[1
]}

Shi, Nongnong ^{[1
]}

Wang, Huizhong ^{[1
]}

Jin, Mingfei ^{[2
,3
,7
]}

Zhong, Silin ^{[8
]}

Fan, Zaifeng ^{[4
,5
]}

Liu, Yule ^{[9
]}

Wu, Zirong ^{[7
]}

Jackson, Stephen ^{[2
,3
]}

Giovannoni, James J. ^{[8
]}

Rolin, Dominique ^{[10
]}

Gallusci, Philippe ^{[10
]}

Hong, Yiguo ^{[1
,2
,3
]}

机构：

[1] Hangzhou Normal Univ, Coll Life & Environm Sci, Res Ctr Plant RNA Signalling, Hangzhou 310036, Zhejiang, Peoples R China

[2] Univ Warwick, Warwick HRI, Warwick CV35 9EF, England

[3] Univ Warwick, Sch Life Sci, Warwick CV35 9EF, England

[4] China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China

[5] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

[6] Chengdu Rongsheng Pharmaceut, Chengdu 610041, Peoples R China

[7] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

[8] Cornell Univ, Boyce Thompson Inst Plant Sci Res, Ithaca, NY 14853 USA

[9] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

[10] Univ Bordeaux 1, UMR Biol Fruit & Pathol 1332, F-33883 Bordeaux, France

来源：

SCIENTIFIC REPORTS | 2012年 / 2卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

CELL-TO-CELL; EXPRESSION; PROTEIN; PLANTS; SUPPRESSION; REGULATOR; INTERACTS; SPREAD;

D O I：

10.1038/srep00836

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants in order to understand the function of plant genes is not well documented. Here we exploit Potato virus X as a tool for virus-induced gene complementation (VIGC). Using VIGC in tomato, we demonstrated that ectopic viral expression of LeMADS-RIN, which encodes a MADS-box transcription factor (TF), resulted in functional complementation of the non-ripening rin mutant phenotype and caused fruits to ripen. Comparative gene expression analysis indicated that LeMADS-RIN up-regulated expression of the SBP-box (SQUAMOSA promoter binding protein-like) gene LeSPL-CNR, but down-regulated the expression of LeHB-1, an HD-Zip homeobox TF gene. Our data support the hypothesis that a transcriptional network may exist among key TFs in the modulation of fruit ripening in tomato.

引用

页数：7

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