Immunization with cDNA of a novel P-III type metalloproteinase from the rattlesnake Crotalus durissus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom

被引：23

作者：

Azofeifa-Cordero, Gabriela ^{[1
,2
]}

Arce-Estradaa, Viviana ^{[1
]}

Flores-Diaz, Marietta ^{[1
]}

Alape-Giron, Alberto ^{[1
,2
]}

机构：

[1] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose 2060, Costa Rica

[2] Univ Costa Rica, Escuela Med, Dept Bioquim, San Jose 2060, Costa Rica

来源：

TOXICON | 2008年 / 52卷 / 02期

关键词：

DNA immunization; Gene Gun; therapeutic antivenom;

D O I：

10.1016/j.toxicon.2008.05.023

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Zinc-dependent metalloproteinases play a key role in the hemorrhage induced by viperid bite envenoming. in this work we report the cloning and sequencing of the cDNA from a novel P-III type metalloprotemase from Crotalus durissus durissus venom glands. The recombinant plasmid was used for DNA immunization in mice using accelerate DNA-coated microparticles with the Gene Gun system. The results showed that there is no significant difference in the efficiency of the immunization in mice when gold or tungsten microparticles were used. A pool of the sera from mice immunized with the metalloprotemase encoding DNA neutralized the hemorrhagic activity of C. d. durissus venom. The coimmunization with DNA encoding the metalloproteinase and a plasmid encoding the murine IL-2 increased the number of mice which show a specific antibody response towards C d. durissus venom antigens. The neutralizing ability of the produced antibodies demonstrates that DNA immunization with tungsten microparticles may be used in strategies for antivenom production. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：302 / 308

页数：7

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