In Vitro Uses of Human Pluripotent Stem Cell-Derived Cardiomyocytes

被引：16
作者：
Matsa, Elena [1 ]
Denning, Chris [1 ]
机构：
[1] Univ Nottingham, Wolfson Ctr Stem Cells Tissue Engn & Modelling ST, Nottingham NG7 2RD, England
来源：
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH | 2012年 / 5卷 / 05期
基金：
英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国工程与自然科学研究理事会;
关键词：
Human pluripotent stem cells; Cardiomyocytes; Drug screening; Toxicology; Safety pharmacology; Disease modelling; Genetic manipulation; GENETIC CORRECTION; HUMAN FIBROBLASTS; DRUG DISCOVERY; SELF-RENEWAL; IPS CELLS; DIFFERENTIATION; LINES; GENERATION; MOUSE; INDUCTION;
D O I：
10.1007/s12265-012-9376-5
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Functional cardiomyocytes can be efficiently derived from human pluripotent stem cells (hPSCs), which collectively include embryonic and induced pluripotent stem cells. This cellular platform presents exciting new opportunities for development of pharmacologically relevant in vitro screens to detect cardiotoxicity, validate novel drug candidates in preclinical trials and understand complex congenital cardiovascular disorders, to advance current clinical therapies. Here, we discuss the progress and impediments the field has faced in using hPSC-derived cardiomyocytes for these in vitro applications, and highlight that rigorous protocol optimisation and standardisation, scalability and automation are remaining obstacles for the generation of pure, mature and clinically relevant hPSC cardiomyocytes.
引用
收藏
页码：581 / 592
页数：12
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