PCR technique application in Yersinia enterocolitica detection in non-inspected swine

被引：4

作者：

Teodoro, VAM ^{[1
]}

Pinto, PSA ^{[1
]}

Vanetti, MCD ^{[1
]}

Bevilacqua, PD ^{[1
]}

Moraes, MP ^{[1
]}

Pinto, MS ^{[1
]}

机构：

[1] UFV, Dept Vet, BR-36570000 Vicosa, MG, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2006年 / 58卷 / 01期

关键词：

swine; contamination; Yersinia enterocolitica; PCR; HACCP;

D O I：

10.1590/S0102-09352006000100002

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The contamination of swine carcasses and tonsils by Yersinia enterocolitica in slaughterhouses without inspection was evaluated. The conventional microbiological analysis was compared with the PCR technique of carcass or tonsil swabs, as a subsidy to the microbiological evaluation in the HACCP system. The costs of the two techniques were also calculated. Y. enterocolitica was not detected by the conventional microbiological analysis. Using the PCR, it was possible to detect this bacterium in carcass (40%) and tonsil (43%) samples. There was no difference between the positive results for the carcass and tonsil samples. The PCR showed to be a more effective, fast and economic alternative for the Y. enterocolitica detection, as compared to the conventional microbiological analysis.

引用

页码：9 / 14

页数：6

共 21 条

[1] Rapid duplex PCR assay for the detection of pathogenic Yersinia enterocolitica strains [J].