A Miniature Graphene-based Biosensor for Intracellular Glucose Measurements

被引：30

作者：

ul Hasan, Kamran ^{[1
,2
]}

Asif, Muhammad H. ^{[1
,3
]}

Hassan, Muhammad Umair ^{[4
]}

Sandberg, Mats O. ^{[5
]}

Nur, O. ^{[1
]}

Willander, M. ^{[1
]}

Fagerholm, Siri ^{[6
]}

Stralfors, Peter ^{[6
]}

机构：

[1] Linkoping Univ, Dept Sci & Technol, SE-60174 Norrkoping, Sweden

[2] CESAT, Islamabad, Pakistan

[3] COMSATS Inst Informat Technol, Dept Phys, Mat Res Lab, Lahore 54000, Pakistan

[4] COMSATS Inst Informat Technol, Dept Phys, CMND, Islamabad 44000, Pakistan

[5] Acreo AB, SE-60221 Norrkoping, Sweden

[6] Linkoping Univ, Dept Clin & Expt Med, SE-58185 Linkoping, Sweden

来源：

ELECTROCHIMICA ACTA | 2015年 / 174卷

关键词：

Graphene; Bio-sensors; Glucose-oxidase; Intracellular sensors; Graphene oxide; Glucose Sensor; ELECTROCHEMICAL BIOSENSORS; SINGLE-LAYER; SENSOR; NANOPARTICLES; NANOSHEETS; ELECTRODE; OXIDASE;

D O I：

10.1016/j.electacta.2015.06.035

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We report on a small and simple graphene-based potentiometric sensor for the measurement of intracellular glucose concentration. A fine borosilicate glass capillary coated with graphene and subsequently immobilized with glucose oxidase (GOD) enzyme is inserted into the intracellular environment of a single human cell. The functional groups on the edge plane of graphene assist the attachment with the free amine terminals of GOD enzyme, resulting in a better immobilization. The sensor exhibits a glucose-dependent electrochemical potential against an Ag/AgCl reference microelectrode which is linear across the whole concentration range of interest (10 - 1000 mu M). Glucose concentration in human fat cell measured by our graphene-based sensor is in good agreement with nuclear magnetic resonance (NMR) spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：574 / 580

页数：7

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